Ilgen C L, Burkholder P M
Immunology. 1974 Jan;26(1):197-203.
Cells which synthesize the fourth component of complement have been isolated from guinea-pig liver through rate zonal density gradient centrifugation of suspensions of liver cells on continuous density gradients (2 to 15 per cent) of Ficoll. This procedure separates non-parenchymal cells from parenchymal cells on the basis of size. Cells which synthesized C4 were identified as low density, non-parenchymal cells which comprised only a small proportion of the cells added to the gradient. Phagocytic cells were restricted to the same portion of the gradient as cells which synthesized C4, and there is evidence that cells which synthesize C4 are also phagocytic.
通过在连续密度梯度（2%至15%）的菲可溶液上对肝细胞悬液进行速率区带密度梯度离心，已从豚鼠肝脏中分离出合成补体第四成分的细胞。该程序根据大小将非实质细胞与实质细胞分离。合成C4的细胞被鉴定为低密度非实质细胞，它们仅占添加到梯度中的细胞的一小部分。吞噬细胞与合成C4的细胞局限于梯度的同一部分，并且有证据表明合成C4的细胞也是吞噬细胞。
