选定细菌的定量红外光分析

Quantitative infrared photoanalysis of selected bacteria.

作者信息

Dugan D L, Wright D N

出版信息

Appl Microbiol. 1974 Aug;28(2):205-11. doi: 10.1128/am.28.2.205-211.1974.

DOI:10.1128/am.28.2.205-211.1974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC186687/

Abstract

A technique to measure transmitted infrared radiation from minute biological systems is described. Infrared color film was exposed by radiation transmitted through bacterial colonies. The resultant photographic image was unique for each species of bacteria examined and spectral analysis of the image provided differential light emission patterns which could be quantitated. A formula for developing numerical comparisons among bacterial colonies was provided. The results of this numerical procedure gave quantitative relationships for the total infrared data from each microbial colony and made possible the differential identification of ten species of medically significant bacteria.

摘要

本文描述了一种测量微小生物系统透射红外辐射的技术。红外彩色胶片通过透过细菌菌落的辐射进行曝光。所得的照片图像对于所检测的每种细菌都是独特的，并且对该图像的光谱分析提供了可定量的不同发光模式。给出了一种用于在细菌菌落之间进行数值比较的公式。该数值程序的结果给出了每个微生物菌落的总红外数据的定量关系，并使得对十种具有医学意义的细菌进行鉴别成为可能。

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