Hermann A, Gorman A L
Neurosci Lett. 1979 Apr;12(1):87-92. doi: 10.1016/0304-3940(79)91485-x.
Ca-dependent and voltage-dependent K+ currents in Aplysia pacemaker neurons were studied separately by electrophoretic injections of Ca2+ ions into the cells to induce the Ca-dependent component and by step depolarizations in the absence of external Ca2+ ions to induce the voltage-dependent component. Intracellular tetraethylammonium (TEA) blocks the voltage-dependent but not the Ca-dependent K+ current. Extracellular TEA blocks both components reversibly but is more effective in inhibiting the Ca-dependent K+ currents. Our results suggest that there are TEA receptor sites for the voltage-dependent K+ current on each side of the membrane, but only an external receptor site for the Ca-dependent K+ current.
通过向海兔起搏神经元中电泳注入Ca2+离子以诱导钙依赖性成分，以及在无细胞外Ca2+离子的情况下进行阶跃去极化以诱导电压依赖性成分，分别对海兔起搏神经元中钙依赖性和电压依赖性钾电流进行了研究。细胞内四乙铵（TEA）阻断电压依赖性钾电流，但不阻断钙依赖性钾电流。细胞外TEA可逆地阻断这两种成分，但在抑制钙依赖性钾电流方面更有效。我们的结果表明，膜两侧均存在电压依赖性钾电流的TEA受体位点，但钙依赖性钾电流仅存在细胞外受体位点。
