“棕色和蓝色反应”用于追踪用辣根过氧化物酶进行细胞内注射的细胞的神经元突起的比较有效性。

The comparative effectiveness of the "brown and blue reactions" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.

作者信息

Deschênes M, Landry P, Labelle A

出版信息

Neurosci Lett. 1979 Apr;12(1):9-15. doi: 10.1016/0304-3940(79)91472-1.

Abstract

The comparative advantages and disadvantages of diaminobenzidine (DAB) and benzidine di--HCl (BDHC) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (HRP) have been studied. The DAB reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. The BDHC procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. This last procedure is, however, much more appropriate for tracing the axonal system of injected cells.

摘要

对二氨基联苯胺（DAB）和盐酸联苯胺（BDHC）用于追踪经辣根过氧化物酶（HRP）细胞内注射过程的比较优势和劣势进行了研究。DAB反应能很好地界定细胞膜，似乎更适合于观察树突的精细结构。BDHC方法更敏感，会诱导晶体形成，填充密集的过程看起来呈磨砂状。然而，后一种方法更适合于追踪注射细胞的轴突系统。