Schipper J, Tilders F J
Neurosci Lett. 1979 May;12(2-3):229-34. doi: 10.1016/0304-3940(79)96067-1.
Formaldehyde-induced fluorescence (FIF) of noradrenalin (NA) was studied in histochemical preparations of the rat iris. With the aid of a two-dimensional microfluorimetric scanning method, quantitative information was obtained from neuronal and non-neuronal elements selectively. Spectral analysis and fluorimetric studies showed the existence of extraneuronal NA in the iris of untreated rats. Surgical and pharmacological (reserpine, amphetamine, imipramine, nialamide) manipulation of sympathetic neurotransmission-induced changes in extraneuronal fluorescence, which did not parallel changes in fluorescence of neuronal elements. A positive relationship was found between changes in extraneuronal fluorescence and the postsynaptic effect (pupil diameter). We hypothesize that extraneuronal catecholamine (CA) fluorescence is a histochemical parameter for synaptic neurotransmission.
在大鼠虹膜的组织化学制剂中研究了去甲肾上腺素（NA）的甲醛诱导荧光（FIF）。借助二维显微荧光扫描方法，选择性地从神经元和非神经元成分中获得了定量信息。光谱分析和荧光研究表明，未处理大鼠的虹膜中存在细胞外NA。交感神经传递的手术和药理学（利血平、苯丙胺、丙咪嗪、尼亚酰胺）操作引起细胞外荧光的变化，这与神经元成分荧光的变化并不平行。发现细胞外荧光的变化与突触后效应（瞳孔直径）之间存在正相关关系。我们假设细胞外儿茶酚胺（CA）荧光是突触神经传递的组织化学参数。
