一株具有温度敏感型recA-表型的大肠杆菌K-12突变体的分离与鉴定。
Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
作者信息
Lloyd R G, Low B, Godson G N, Birge E A
出版信息
J Bacteriol. 1974 Oct;120(1):407-15. doi: 10.1128/jb.120.1.407-415.1974.
Abstract
A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.
摘要
在一株在高温下无法生长的PolA(-)菌株的衍生物中，鉴定出一种导致温度敏感型RecA(-)表型的突变。该突变等位基因（recA200）被证明与cysC连锁，在35至42摄氏度范围内赋予明显的温度敏感型、紫外线敏感型Rec(-)表型，并且在杂交实验中，在42摄氏度时与转移recA(-)的高频重组菌株（Hfr）不能互补。对携带recA200和polA的双突变体在限制温度下的生长和合成DNA的能力进行了检测。
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