大肠杆菌对紫外线敏感的诱变基因mutU4，与polA基因不相容（无法存活）。
Ultraviolet-sensitive mutator mutU4 of Escherichia coli inviable with polA.
作者信息
Siegel E C
出版信息
J Bacteriol. 1973 Jan;113(1):161-6. doi: 10.1128/jb.113.1.161-166.1973.
Abstract
Attempts to transduce the ultraviolet-sensitive mutator lesion mutU4 into strains deficient in deoxyribonucleic acid polymerase I (polA) were unsuccessful. Mutator recombinants were found when the polA recipient had first been reverted to Pol(+) by selection for resistance to methyl methanesulfonate. The inviability of the mutU4 polA double mutant was demonstrated by a reduction in the absolute number of transductants when the recipient was polA as compared with Pol(+), and selection was made for markers very close to mutU4. Double mutants containing mutU4 and polA4, which determines a cold-sensitive polymerase, were unable to grow at 24 C, the nonpermissive temperature.
摘要
将对紫外线敏感的诱变损伤mutU4导入缺乏脱氧核糖核酸聚合酶I（polA）的菌株的尝试未成功。当通过选择对甲磺酸甲酯的抗性使polA受体首先回复为Pol（+）时，发现了诱变重组体。当受体为polA时，与Pol（+）相比，转导子的绝对数量减少，这证明了mutU4 polA双突变体的不可存活性，并对非常接近mutU4的标记进行了选择。含有mutU4和polA4（决定一种冷敏感聚合酶）的双突变体在非允许温度24℃下无法生长。
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