检测小鼠对异种移植免疫反应不同方面的两种方法。
Two methods for detecting different aspects of the immune response to a xenograft in mice.
作者信息
Newlands E S
出版信息
Immunology. 1974 Oct;27(4):543-52.
Two radiolabel assays of immunity to a xenograft in mice are described. One detects definite cell death while the other, which is more sensitive, detects both cell death and the inhibition of DNA turnover. It has been possible to demonstrate different cell populations in response to subcutaneous immunization. Complement-mediated cytotoxicity and the killing of antibody-coated target cells can be readily measured. The importance of the particular assay used and the tissue of origin of the effector cells in detecting different parameters of the immune responses is emphasized.
本文描述了两种用于检测小鼠对异种移植免疫的放射性标记检测方法。一种方法可检测明确的细胞死亡，而另一种更灵敏的方法既能检测细胞死亡，又能检测DNA周转的抑制情况。皮下免疫后，已能够证明存在不同的细胞群体。补体介导的细胞毒性以及抗体包被靶细胞的杀伤作用都可以很容易地检测出来。文中强调了所使用的特定检测方法以及效应细胞的组织来源在检测免疫反应不同参数方面的重要性。
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