产大肠杆菌素因子E1 DNA的复制：关于不连续复制机制的证据
Replication of colicinogenic factor E 1 DNA: evidence for a discontinuous replication mechanism.
作者信息
Staudenbauer W L
出版信息
Nucleic Acids Res. 1974 Sep;1(9):1153-64. doi: 10.1093/nar/1.9.1153.
Abstract
The mechanism of Col E 1 DNA replication was investigated in a plasmolysed cell system prepared from chloramphenicoltreated E. coli JC 411 (Col E 1). After pulse-labelling with (3)H-dTTP a considerable fraction of the newly synthesized DNA was recovered as single-stranded fragments. Upon alkali denaturation the pulse label was found in DNA chains sedimenting slower than unit length Col E 1 strands with a prominent peak at 5 S. During a chase with unlabeled precursors the label is transferred nearly completely into supercoiled Col E 1 DNA. DNA ligase appears to be required for the joining of the 5 S pieces since in the absence of NAD an accumulation of short fragments is observed.
摘要
在由氯霉素处理的大肠杆菌JC 411（Col E 1）制备的质壁分离细胞系统中研究了Col E 1 DNA复制机制。用（3）H-dTTP进行脉冲标记后，相当一部分新合成的DNA以单链片段形式回收。碱变性后，发现脉冲标记存在于沉降速度比单位长度Col E 1链慢的DNA链中，在5 S处有一个突出的峰。在用未标记的前体进行追踪过程中，标记几乎完全转移到超螺旋Col E 1 DNA中。DNA连接酶似乎是连接5 S片段所必需的，因为在没有NAD的情况下会观察到短片段的积累。
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