通过亲和层析法纯化兔抗精胺抗血清。

Purification of rabbit antispermine antiserum by affinity chromatography.

作者信息

Bartos F, Bartos D, Campbell R A, Grettie D P, McDaniel P, Davis M, Smejtek P

出版信息

Res Commun Chem Pathol Pharmacol. 1979 Mar;23(3):547-59.

PMID:461975

Abstract

Immunoglobulins G were isolated from a crude rabbit antispermine antiserum using a protein A-Sepharose CL-4B column. Affinity chromatography coupling spermine to the Sepharose matrix was employed for separation of antispermine antibodies from these immunoglobulins. Four fractions of antispermine antibodies were isolated by stepwise elution with solutions at pH range from 4 to 1. Binding constants were determined and cross-reactivity with other polyamines was tested for each fraction of antibodies. Highly specific antispermine antibodies were found in fraction IV.

摘要

使用蛋白A-琼脂糖CL-4B柱从粗制兔抗精胺抗血清中分离免疫球蛋白G。采用将精胺偶联到琼脂糖基质上的亲和色谱法从这些免疫球蛋白中分离抗精胺抗体。通过用pH范围为4至1的溶液逐步洗脱，分离出四部分抗精胺抗体。测定结合常数，并测试各部分抗体与其他多胺的交叉反应性。在第四部分中发现了高度特异性的抗精胺抗体。