类风湿性关节炎滑膜组织洗脱液将胸苷降解为胸腺嘧啶。
Degradation of thymidine to thymine by rheumatoid arthritis synovial tissue eluate.
作者信息
Nykänen P J
出版信息
Scand J Immunol. 1979;9(5):477-82. doi: 10.1111/j.1365-3083.1979.tb03070.x.
A study was made of the effect of rheumatoid synovial tissue eluate on phytohaemagglutinin (PHA) stimulation of peripheral blood lymphocytes. It was found that rheumatoid synovial tissue eluate caused marked inhibition of 3H-thymidine incorporation by lymphocytes stimulated with PHA. Synovial tissue from traumatic joints had no effect. The inhibitor was a heat-sensitive and nondialysable substance. The inhibitory effect of PHA stimulation was diminished by thymine. In thin-layer chromatography it showed the capacity to convert thymidine to thymine. Because thymine is not incorporated by DNA-synthesizing cells, this report explains why negative results are obtained when lymphocytes are stimulated by rheumatoid synovial tissue using 3H-thymidine incorporation as an indicator of lymphocyte activation.
研究了类风湿性滑膜组织洗脱液对植物血凝素（PHA）刺激外周血淋巴细胞的影响。发现类风湿性滑膜组织洗脱液可显著抑制PHA刺激的淋巴细胞对3H-胸腺嘧啶核苷的掺入。创伤性关节的滑膜组织则无此作用。该抑制剂是一种热敏感且不可透析的物质。胸腺嘧啶可减弱PHA刺激的抑制作用。在薄层色谱分析中，它显示出将胸腺嘧啶核苷转化为胸腺嘧啶的能力。由于DNA合成细胞不掺入胸腺嘧啶，本报告解释了为何以3H-胸腺嘧啶核苷掺入作为淋巴细胞活化指标，用类风湿性滑膜组织刺激淋巴细胞时会得到阴性结果。
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