Pendleton I R, Kim K S, Bernheimer A W
J Bacteriol. 1972 May;110(2):722-30. doi: 10.1128/jb.110.2.722-730.1972.
A method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. Membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. A similar antigen-antibody system, streptolysin O-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. There was an uneven distribution of ferritin in erythrocyte membranes suggesting that the distribution of cholesterol may not be entirely random. Mycoplasma gallisepticum was intensely labeled, but Acholeplasma laidlawii with or without cholesterol in the membranes was not labeled, suggesting an unusual location for cholesterol in A. laidlawii membranes. As controls, two of three species of bacterial membranes lacking cholesterol were not ferritin-labeled.
描述了一种通过铁蛋白标记技术检测红细胞、支原体和细菌细胞膜中胆固醇的方法。用cereolysin（一种特异性结合胆固醇的细菌溶血素）处理细胞膜，然后用铁蛋白 - 抗破伤风溶血素处理，细胞膜会被胆固醇特异性地铁蛋白标记。一种类似的抗原 - 抗体系统，即链球菌溶血素O - 铁蛋白 - 抗链球菌溶血素，也成功用于红细胞膜。铁蛋白在红细胞膜中的分布不均匀，这表明胆固醇的分布可能并非完全随机。鸡毒支原体被强烈标记，但膜中有无胆固醇的莱氏无胆甾原体均未被标记，这表明胆固醇在莱氏无胆甾原体膜中的位置不寻常。作为对照，三种缺乏胆固醇的细菌膜中有两种未被铁蛋白标记。
