Larsen S A, Jones W L
Appl Microbiol. 1972 Jul;24(1):101-7. doi: 10.1128/am.24.1.101-107.1972.
Human sera from patients with culturally confirmed listeriosis were tested for immunoglobulin M (IgM) and immunoglobulin G (IgG) agglutinating antibodies with trypsinized antigens of Listeria monocytogenes, Streptococcus faecalis, and Staphylococcus aureus. The response of humans to listeria infections is mainly IgM rather than IgG as found in animals. The antigens prepared from L. monocytogenes serotypes 1a, 1b, 2, 4b, and 4d were evaluated for specificity with normal sera, sera from patients with various other diseases, and sera from patients with listeriosis. The trypsinized antigens appeared to be specific for listeria antibodies with a cross-reaction rate of from 5.4 to 6%. Cross-reaction with S. aureus can be eliminated by absorption of the serum with S. aureus. This agglutination technique appears to be applicable for diagnostic testing, but, as with all serological procedures, both acute and convalescent sera should be tested.
对经培养确诊为李斯特菌病患者的人血清，用经胰蛋白酶处理的单核细胞增生李斯特菌、粪肠球菌和金黄色葡萄球菌抗原检测免疫球蛋白M（IgM）和免疫球蛋白G（IgG）凝集抗体。人类对李斯特菌感染的反应主要是IgM，而非如在动物中发现的IgG。对由1a、1b、2、4b和4d血清型的单核细胞增生李斯特菌制备的抗原，用正常血清、患有各种其他疾病患者的血清以及患有李斯特菌病患者的血清评估其特异性。经胰蛋白酶处理的抗原似乎对李斯特菌抗体具有特异性，交叉反应率为5.4%至6%。血清与金黄色葡萄球菌的交叉反应可通过用金黄色葡萄球菌吸收血清来消除。这种凝集技术似乎适用于诊断检测，但与所有血清学程序一样，应同时检测急性期和恢复期血清。
