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定量痰培养作为常规微生物实验室排除假阳性报告的一种方法。

Quantitative sputum culture as a means of excluding false positive reports in the routine microbiology laboratory.

作者信息

Wilson M J, Martin D E

出版信息

J Clin Pathol. 1972 Aug;25(8):697-700. doi: 10.1136/jcp.25.8.697.

DOI:10.1136/jcp.25.8.697
PMID:4627746
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC477479/
Abstract

A relatively simple technique for sputum homogenization and dilution is described. Results show that this technique is reliable for isolation and quantitative culture of organisms from cases of chest infection. The technique has resulted in significant reduction in false positive reports, particularly when a system of interpretative reporting is utilized based on 10(7) organisms per millilitre of sputum being accepted as evidence of significant infection.

摘要

本文描述了一种相对简单的痰液匀化和稀释技术。结果表明,该技术对于从胸部感染病例中分离和定量培养微生物是可靠的。该技术显著减少了假阳性报告,特别是当采用基于每毫升痰液中10⁷个微生物被视为严重感染证据的解释性报告系统时。

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本文引用的文献

1
Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic.菌尿症与尿路感染的诊断;关于蛋氨酸作为尿路防腐剂应用的观察
AMA Arch Intern Med. 1957 Nov;100(5):709-14. doi: 10.1001/archinte.1957.00260110025004.
2
Diagnostic and therapeutic advantages of serial quantitative cultures of fresh sputum in acute bacterial pneumonia.急性细菌性肺炎中新鲜痰液连续定量培养的诊断和治疗优势
Am Rev Respir Dis. 1969 Dec;100(6):831-8. doi: 10.1164/arrd.1969.100.6.831.
3
Quantitation of microorganisms in sputum.痰液中微生物的定量分析。
Appl Microbiol. 1969 Aug;18(2):214-20. doi: 10.1128/am.18.2.214-220.1969.
4
Value of dilute inocula in cultural examination of sputum.痰液培养检查中稀释接种物的价值
Lancet. 1965 Nov 20;2(7421):1046-8. doi: 10.1016/s0140-6736(65)90572-6.