Giacobini E
J Neurosci Res. 1975;1(1):1-18. doi: 10.1002/jnr.490010102.
The author reviews available microtechniques and results on cellular analysis of single neurons emphasizing the identification of putative neurotransmitter molecules. Highly sensitive microquantitative methods for identification and assay of known and putative neurotransmitters have been developed within the last 15 years. Several of the methods reported in the article fulfill the requirements of both specificity and sensitivity for exploring and measuring the level of transmitter molecules in single neurons. Some of the available techniques, mostly those which associate GC or TLC with MS, have been able to unambiguously demonstrate the presence of putative neurotransmitter molecules, or at least of substances which are strongly suspected of fulfilling a functional role in the nervous system.
作者回顾了现有的显微技术以及对单个神经元进行细胞分析的结果，重点强调了对假定神经递质分子的鉴定。在过去15年中，已经开发出了用于鉴定和测定已知及假定神经递质的高灵敏度微量定量方法。文章中报道的几种方法满足了探索和测量单个神经元中递质分子水平的特异性和灵敏度要求。一些现有的技术，主要是那些将气相色谱（GC）或薄层色谱（TLC）与质谱（MS）相结合的技术，已经能够明确证明假定神经递质分子的存在，或者至少证明那些被强烈怀疑在神经系统中发挥功能作用的物质的存在。
