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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1972 Jun;128(2):415-20. doi: 10.1042/bj1280415.

The modification of pyruvate kinase activity in vitro was examined by altering the environmental [Mg(2+)]/[Ca(2+)] ratio with EDTA on the one hand and isolated rat liver mitochondria on the other. 2. Controlled additions of Ca(2+) and EDTA caused pyruvate kinase activity to be alternately and rapidly switched on and off. 3. By being able to accumulate Ca(2+) in preference to Mg(2+) rat liver mitochondria were able to alter the [Mg(2+)]/[Ca(2+)] ratio in the vicinity of pyruvate kinase and thereby modify the activity of this enzyme. 4. The possible role of mitochondria in modifying pyruvate kinase and other ion-sensitive cytoplasmic enzyme activities is discussed.

一方面通过用乙二胺四乙酸（EDTA）改变环境中的[Mg(2+)]/[Ca(2+)]比值，另一方面使用分离的大鼠肝脏线粒体，来研究丙酮酸激酶活性在体外的修饰情况。2. 控制性地添加Ca(2+)和EDTA会使丙酮酸激酶活性交替且快速地开启和关闭。3. 大鼠肝脏线粒体能够优先积累Ca(2+)而非Mg(2+)，从而能够改变丙酮酸激酶附近的[Mg(2+)]/[Ca(2+)]比值，进而修饰该酶的活性。4. 讨论了线粒体在修饰丙酮酸激酶和其他离子敏感型细胞质酶活性方面可能发挥的作用。

Biochem J. 1972 Jun;128(2):415-20. doi: 10.1042/bj1280415.

The modification of pyruvate kinase activity in vitro was examined by altering the environmental [Mg(2+)]/[Ca(2+)] ratio with EDTA on the one hand and isolated rat liver mitochondria on the other. 2. Controlled additions of Ca(2+) and EDTA caused pyruvate kinase activity to be alternately and rapidly switched on and off. 3. By being able to accumulate Ca(2+) in preference to Mg(2+) rat liver mitochondria were able to alter the [Mg(2+)]/[Ca(2+)] ratio in the vicinity of pyruvate kinase and thereby modify the activity of this enzyme. 4. The possible role of mitochondria in modifying pyruvate kinase and other ion-sensitive cytoplasmic enzyme activities is discussed.

一方面通过用乙二胺四乙酸（EDTA）改变环境中的[Mg(2+)]/[Ca(2+)]比值，另一方面使用分离的大鼠肝脏线粒体，来研究丙酮酸激酶活性在体外的修饰情况。2. 控制性地添加Ca(2+)和EDTA会使丙酮酸激酶活性交替且快速地开启和关闭。3. 大鼠肝脏线粒体能够优先积累Ca(2+)而非Mg(2+)，从而能够改变丙酮酸激酶附近的[Mg(2+)]/[Ca(2+)]比值，进而修饰该酶的活性。4. 讨论了线粒体在修饰丙酮酸激酶和其他离子敏感型细胞质酶活性方面可能发挥的作用。