Saif L J, Bohl E H, Gupta R K
Infect Immun. 1972 Oct;6(4):600-9. doi: 10.1128/iai.6.4.600-609.1972.
The porcine immunoglobulins M (IgM), A (IgA), and G (IgG) were isolated and purified and some of the properties of the porcine milk IgA were examined. Monospecific antisera which were prepared against these immunoglobulins in rabbits were then used to absorb a particular class of immunoglobulin from sow serum, colostrum, and milk in an attempt to identify the immunoglobulin classes of neutralizing antibodies to the porcine enteric virus, transmissible gastroenteritis (TGE). The results of these absorption studies suggest that in colostrum and milk from sows experimentally (orally) or naturally infected with live virulent TGE virus, IgA is the predominant immunoglobulin class of TGE antibodies. Both IgA and IgG TGE antibodies appeared to be present in the serum from these sows, but with IgG TGE antibodies predominating. In contrast, in the serum, colostrum and milk from sows vaccinated intramuscularly or intramammarily with live attenuated TGE virus, the TGE antibody activity was associated mainly with the IgG class of immunoglobulins. These results provide additional data indicating that the route of infection or vaccination markedly influences the immunoglobulin class of antibodies in colostrum and milk. Secondly, IgA antibodies in mammary secretions are probably essential for providing optimal passive immunity of nursing pigs against infection with TGE virus.
分离并纯化了猪免疫球蛋白M（IgM）、A（IgA）和G（IgG），并检测了猪乳IgA的一些特性。然后用在兔体内制备的针对这些免疫球蛋白的单特异性抗血清，从母猪血清、初乳和乳汁中吸收特定类别的免疫球蛋白，试图鉴定针对猪肠道病毒——传染性胃肠炎（TGE）的中和抗体的免疫球蛋白类别。这些吸收研究结果表明，在经实验性（口服）或自然感染强毒TGE病毒的母猪的初乳和乳汁中，IgA是TGE抗体的主要免疫球蛋白类别。这些母猪的血清中似乎同时存在IgA和IgG TGE抗体，但以IgG TGE抗体为主。相比之下，在经肌肉注射或乳腺内接种弱毒TGE病毒的母猪的血清、初乳和乳汁中，TGE抗体活性主要与IgG类免疫球蛋白相关。这些结果提供了更多数据，表明感染或接种途径显著影响初乳和乳汁中抗体的免疫球蛋白类别。其次，乳腺分泌物中的IgA抗体可能对于为哺乳仔猪提供针对TGE病毒感染的最佳被动免疫至关重要。
