通过组织培养中的免疫荧光法测定狂犬病抗体
Rabies antibody determination by immunofluorescence in tissue culture.
作者信息
Debbie J G, Andrulonis J A, Abelseth M K
出版信息
Infect Immun. 1972 Jun;5(6):902-4. doi: 10.1128/iai.5.6.902-904.1972.
Abstract
A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of African green monkey (Vero) and on BHK-21 cells was investigated to determine its utilization in a TC-FA neutralization procedure. Although both the HEP and ERA viruses infected Vero and BHK-21 cells, the amount of fluorescent antigen observed was most consistent with ERA virus and BHK-21 cells.
摘要
一种用于测量狂犬病中和抗体的组织培养（TC）-荧光抗体（FA）技术被发现是可靠的，并且与标准的小鼠血清中和试验相当。该试验是在Lab-Tek TC培养皿载玻片上用感染ERA疫苗病毒株的BHK-21细胞进行的。对在非洲绿猴（Vero）连续细胞系和BHK-21细胞上生长的Flury高代鸡胚传代（HEP）狂犬病病毒株进行了研究，以确定其在TC-FA中和程序中的应用。虽然HEP病毒和ERA病毒都能感染Vero细胞和BHK-21细胞，但观察到的荧光抗原量与ERA病毒和BHK-21细胞最为一致。
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本文引用的文献
1
An Attenuated Rabies Vaccine for Domestic Animals Produced in Tissue Culture.一种在组织培养中生产的用于家畜的减毒狂犬病疫苗。
Can Vet J. 1964 Nov;5(11):279-86.
2
Pantropism of rabies virus in free-ranging rabid red fox Vulpes fulva.狂犬病病毒在野生狂犬病赤狐（Vulpes fulva）中的泛嗜性。
J Wildl Dis. 1970 Oct;6(4):500-6. doi: 10.7589/0090-3558-6.4.500.
3
ENDOSYMBIOTIC RELATIONSHIP BETWEEN ANIMAL VIRUSES AND HOST CELLS. A STUDY OF RABIES VIRUS IN TISSUE CULTURE.动物病毒与宿主细胞之间的内共生关系。狂犬病病毒在组织培养中的研究。
J Exp Med. 1964 Dec 1;120(6):1099-116. doi: 10.1084/jem.120.6.1099.
4
CULTIVATION OF RABIES VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38.狂犬病病毒在人二倍体细胞株WI-38中的培养
J Immunol. 1964 Sep;93:353-66.
5
MECHANISM OF THE CYTOPATHIC EFFECT OF RABIES VIRUS IN TISSUE CULTURE.狂犬病病毒在组织培养中的细胞病变效应机制
Virology. 1963 Sep;21:128-31. doi: 10.1016/0042-6822(63)90312-x.
6
Biological modification of rabies virus as a result of its adaptation to chicks and developing chick embryos.狂犬病病毒因适应雏鸡和发育中的鸡胚而发生的生物学修饰。
Bull World Health Organ. 1954;10(5):709-24.
7
Growth characteristics of rabies virus in primary chick embryo cells.
Virology. 1965 Oct;27(2):199-204. doi: 10.1016/0042-6822(65)90160-1.
8
Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle growth curve.聚离子对狂犬病病毒在组织培养中感染性的影响：单周期生长曲线的构建
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J Virol. 1967 Dec;1(6):1224-6. doi: 10.1128/JVI.1.6.1224-1226.1967.
10
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