通过组织培养中的免疫荧光法测定狂犬病抗体
Rabies antibody determination by immunofluorescence in tissue culture.
作者信息
Debbie J G, Andrulonis J A, Abelseth M K
出版信息
Infect Immun. 1972 Jun;5(6):902-4. doi: 10.1128/iai.5.6.902-904.1972.
Abstract
A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of African green monkey (Vero) and on BHK-21 cells was investigated to determine its utilization in a TC-FA neutralization procedure. Although both the HEP and ERA viruses infected Vero and BHK-21 cells, the amount of fluorescent antigen observed was most consistent with ERA virus and BHK-21 cells.
摘要
一种用于测量狂犬病中和抗体的组织培养（TC）-荧光抗体（FA）技术被发现是可靠的，并且与标准的小鼠血清中和试验相当。该试验是在Lab-Tek TC培养皿载玻片上用感染ERA疫苗病毒株的BHK-21细胞进行的。对在非洲绿猴（Vero）连续细胞系和BHK-21细胞上生长的Flury高代鸡胚传代（HEP）狂犬病病毒株进行了研究，以确定其在TC-FA中和程序中的应用。虽然HEP病毒和ERA病毒都能感染Vero细胞和BHK-21细胞，但观察到的荧光抗原量与ERA病毒和BHK-21细胞最为一致。
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