对与葡萄球菌食物中毒有关的食品中肠毒素的血清学检测。
Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning.
作者信息
Gilbert R J, Wieneke A A, Lanser J, Simkovicová M
出版信息
J Hyg (Lond). 1972 Dec;70(4):755-62. doi: 10.1017/s0022172400022592.
Abstract
Two methods are described for the extraction of enterotoxin from foods incriminated in incidents of staphylococcal food poisoning. Enterotoxin was detected serologically in 12 of 24 food samples from 20 separate incidents: eight samples contained enterotoxin A, three contained D and one both A and B. The amount of enterotoxin in nine foods, based on 100% recovery, varied from 0.02 to 0.09 mug./g.Data are also given on the numbers of Staphylococcus aureus isolated from samples of food from 39 food poisoning incidents. Colony counts varied between 7.5 x 10(5) and 9 x 10(9)/g. with a median value of 7 x 10(7)/g.
摘要
文中描述了两种从与葡萄球菌食物中毒事件相关的食品中提取肠毒素的方法。在来自20起不同事件的24份食品样本中，有12份通过血清学检测出了肠毒素：8份样本含有肠毒素A，3份含有D，1份同时含有A和B。基于100%的回收率，9种食品中的肠毒素含量在0.02至0.09微克/克之间。文中还给出了从39起食物中毒事件的食品样本中分离出的金黄色葡萄球菌数量的数据。菌落计数在7.5×10⁵至9×10⁹/克之间，中位数为7×10⁷/克。
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