• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
M band protein. Two components isolated from chicken breast muscle.M带蛋白。从鸡胸肌中分离出的两种成分。
J Cell Biol. 1972 Dec;55(3):681-95. doi: 10.1083/jcb.55.3.681.
2
M-protein.M蛋白
J Biochem. 1974 Feb;75(2):367-80. doi: 10.1093/oxfordjournals.jbchem.a130403.
3
H-protein and X-protein. Two new components of the thick filaments of vertebrate skeletal muscle.H蛋白和X蛋白。脊椎动物骨骼肌粗肌丝的两种新成分。
J Mol Biol. 1983 Nov 5;170(3):675-98. doi: 10.1016/s0022-2836(83)80127-2.
4
Eu-actinin, a new structural protein of the Z-line of striated muscles.真肌动蛋白，一种横纹肌Z线的新型结构蛋白。
J Biochem. 1981 Jan;89(1):297-310. doi: 10.1093/oxfordjournals.jbchem.a133194.
5
A new 220,000 dalton protein located in the Z lines of vertebrate skeletal muscle.一种位于脊椎动物骨骼肌Z线的新型220,000道尔顿蛋白质。
J Biochem. 1981 Jun;89(6):1981-4. doi: 10.1093/oxfordjournals.jbchem.a133401.
6
[Protein makeup of rabbit myofibrils determined by a disc eletrophoretic method in the presence of sodium dodecyl sulfate].[用十二烷基硫酸钠存在下的圆盘电泳法测定兔肌原纤维的蛋白质组成]
Tsitologiia. 1975 Oct;17(10):1148-54.
7
A protein that binds specifically to the M-line of skeletal muscle is identified as the muscle form of creatine kinase.一种与骨骼肌M线特异性结合的蛋白质被鉴定为肌酸激酶的肌肉形式。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):702-5. doi: 10.1073/pnas.70.3.702.
8
Extractability of actin and actin-like protein from myosin-removed myofibrils of skeletal muscle.从去除肌球蛋白的骨骼肌肌原纤维中提取肌动蛋白和肌动蛋白样蛋白的能力。
J Biochem. 1980 Aug;88(2):605-8. doi: 10.1093/oxfordjournals.jbchem.a133009.
9
Preparation of native connectin from chicken breast muscle.从鸡胸肌肉中制备天然肌联蛋白
J Biochem. 1983 Dec;94(6):2083-5. doi: 10.1093/oxfordjournals.jbchem.a134569.
10
Titin: major myofibrillar components of striated muscle.肌联蛋白：横纹肌的主要肌原纤维成分。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3698-702. doi: 10.1073/pnas.76.8.3698.

引用本文的文献

1
Isolation and characterization of distinct domains of sarcolemma and T-tubules from rat skeletal muscle.大鼠骨骼肌肌膜和T小管不同结构域的分离与特性分析
Biochem J. 1995 Apr 1;307 ( Pt 1)(Pt 1):273-80. doi: 10.1042/bj3070273.
2
Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.针对大鼠骨骼肌肌原纤维成分的单克隆抗体可修饰培养细胞的中间丝。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2335-9. doi: 10.1073/pnas.78.4.2335.
3
Biochemical and ultrastructural aspects of Mr 165,000 M-protein in cross-striated chicken muscle.横纹肌鸡肌肉中165,000 Mr M蛋白的生化和超微结构方面
J Cell Biol. 1980 Sep;86(3):775-83. doi: 10.1083/jcb.86.3.775.
4
A quantitative analysis of elastic, entropic, electrostatic, and osmotic forces within relaxed skinned muscle fibers.对松弛的去皮肌纤维内的弹性、熵、静电和渗透力的定量分析。
Biophys Struct Mech. 1980;7(1):17-40. doi: 10.1007/BF00538156.
5
Compositional studies of myofibrils from rabbit striated muscle.兔横纹肌肌原纤维的成分研究。
J Cell Biol. 1976 Jan;68(1):123-41. doi: 10.1083/jcb.68.1.123.
6
N-lines and M-brands in cardiac muscle.心肌中的N线和M带。
Experientia. 1975 Apr 15;31(4):443-4. doi: 10.1007/BF02026368.
7
Comparison of the localization of several muscle proteins in relaxed and contracted myofibrils.几种肌肉蛋白在松弛和收缩肌原纤维中的定位比较。
Experientia. 1978 Jan 15;34(1):38-40. doi: 10.1007/BF01921887.
8
Localization of creatine kinase isoenzymes in myofibrils. I. Chicken skeletal muscle.肌酸激酶同工酶在肌原纤维中的定位。I. 鸡骨骼肌
J Cell Biol. 1977 Nov;75(2 Pt 1):297-317. doi: 10.1083/jcb.75.2.297.

本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
3
[DETERMINATION OF THE LOCALIZATION OF MUSCLE PROTEINS IN ISOLATED MYOFIBRILS WITH THE FLUORESCENT ANTIBODY METHOD].[用荧光抗体法测定分离肌原纤维中肌肉蛋白的定位]
Biofizika. 1963;8:569-74.
4
The distribution of muscle antigens in contracted myofibrils determined by fluorescein-labeled antibodies.用荧光素标记抗体测定收缩肌原纤维中肌肉抗原的分布。
J Biophys Biochem Cytol. 1961 Oct;11(1):67-75. doi: 10.1083/jcb.11.1.67.
5
Some observations on the localization of myosin, actin and tropomyosin in the rabbit myofibril.关于肌球蛋白、肌动蛋白和原肌球蛋白在兔肌原纤维中定位的一些观察结果。
Biochem J. 1958 Jan;68(1):12-7. doi: 10.1042/bj0680012.
6
Extraction of proteins other than myosin from the isolated rabbit myofibril.从分离出的兔肌原纤维中提取肌球蛋白以外的蛋白质。
Biochem J. 1958 Jan;68(1):5-12. doi: 10.1042/bj0680005.
7
II. Separation of antibody-active proteins from various animal sera by ethanol fractionation techniques.二、通过乙醇分级分离技术从各种动物血清中分离抗体活性蛋白。
Methods Med Res. 1952;5:284-300.
8
Electron microscope observations of the location of actin and tropomyosin in the rabbit myofibril.兔肌原纤维中肌动蛋白和原肌球蛋白位置的电子显微镜观察。
J Ultrastruct Res. 1967 Aug;19(3):260-72. doi: 10.1016/s0022-5320(67)80220-x.
9
X-ray diffraction from living striated muscle during contraction.收缩过程中活体横纹肌的X射线衍射
Nature. 1965 Jun 26;206(991):1357-8. doi: 10.1038/2061357a0.
10
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.

M带蛋白。从鸡胸肌中分离出的两种成分。

M band protein. Two components isolated from chicken breast muscle.

作者信息

Eaton B L, Pepe F A

出版信息

J Cell Biol. 1972 Dec;55(3):681-95. doi: 10.1083/jcb.55.3.681.

DOI:10.1083/jcb.55.3.681
PMID:4632583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2108813/
Abstract

M band protein can be specifically extracted from fresh chicken breast muscle myofibrils suspended in 5 mM Tris-HCl pH 8.0. During discontinuous polyacrylamide gel electrophoresis the isolated protein separates into three bands which can be identified as two separate components (A, B) and a complex of the two. When partially purified fractions of the separated components are combined, an increase in the intensity of the band containing the complex can be shown. The polypeptide chain weights of the two components are 100,000 (A) and 40,000 (B) daltons as estimated by sodium dodecyl sulfate- (SDS-) polyacrylamide gel electrophoresis. Antibody prepared against total M band protein stains only the M band of the myofibril and is completely absorbed by M band protein. M band protein also absorbs the M band staining specifically from antibody which stains both I and M bands. Immunodiffusion data indicate that anti-M band is a mixture of two specific antibodies, one against each component.

摘要

M带蛋白可从悬浮于5 mM Tris-HCl（pH 8.0）中的新鲜鸡胸肌肌原纤维中特异性提取。在不连续聚丙烯酰胺凝胶电泳过程中，分离出的蛋白可分为三条带，这三条带可被鉴定为两个单独的组分（A、B）以及二者的复合物。当将分离组分的部分纯化级分混合时，含有复合物的条带强度会增加。通过十二烷基硫酸钠-（SDS-）聚丙烯酰胺凝胶电泳估计，这两个组分的多肽链分子量分别为100,000（A）和40,000（B）道尔顿。针对总M带蛋白制备的抗体仅对肌原纤维的M带染色，并且会被M带蛋白完全吸收。M带蛋白还能特异性地从同时对I带和M带染色的抗体中吸收M带染色。免疫扩散数据表明，抗M带是两种特异性抗体的混合物，每种抗体针对一个组分。