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Isotopic labeling of embryo, yolk sac, and intracellular parasites of the embryonated hen's egg by yolk replacement.通过卵黄置换对胚胎、卵黄囊及鸡胚内寄生虫进行同位素标记。
Appl Microbiol. 1973 Feb;25(2):270-4. doi: 10.1128/am.25.2.270-274.1973.
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Z Tierphysiol Tierernahr Futtermittelkd. 1974 Aug;33(5):250-67.
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本文引用的文献

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The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae.某些盐类、氨基酸、糖类及蛋白质对立克次氏体稳定性的影响。
J Bacteriol. 1950 Apr;59(4):509-22. doi: 10.1128/jb.59.4.509-522.1950.
2
Metabolic studies of rickettsiae. I. The effects of antimicrobial substances and enzyme inhibitors on the oxidation of glutamate by purified rickettsiae.立克次氏体的代谢研究。I. 抗菌物质和酶抑制剂对纯化立克次氏体氧化谷氨酸的影响。
J Immunol. 1951 Aug;67(2):123-36.
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Preparation of lipide extracts from brain tissue.脑组织脂质提取物的制备。
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STAINING RICKETTSIAE IN YOLK-SAC CULTURES.卵黄囊培养物中里克次氏体的染色
Stain Technol. 1964 May;39:135-40. doi: 10.3109/10520296409061219.
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On the chemical production of developmental abnormalities and of phenocopies in chicken embryos.关于鸡胚发育异常和拟表型的化学生产
J Cell Physiol Suppl. 1954 May;43(Suppl. 1):261-305. doi: 10.1002/jcp.1030430412.
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Observations on the hemolytic properties of typhus rickettsiae.关于斑疹伤寒立克次体溶血特性的观察
J Bacteriol. 1954 Jun;67(6):724-30. doi: 10.1128/jb.67.6.724-730.1954.
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Effects of hyperoxia on chick embryos injected with trypan blue.高氧对注射锥虫蓝的鸡胚的影响。
Teratology. 1972 Jun;5(3):315-8. doi: 10.1002/tera.1420050306.

通过卵黄置换对胚胎、卵黄囊及鸡胚内寄生虫进行同位素标记。

Isotopic labeling of embryo, yolk sac, and intracellular parasites of the embryonated hen's egg by yolk replacement.

作者信息

Hurd R A, Winkler H H

出版信息

Appl Microbiol. 1973 Feb;25(2):270-4. doi: 10.1128/am.25.2.270-274.1973.

DOI:10.1128/am.25.2.270-274.1973
PMID:4632853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380785/
Abstract

A technique is described which allows the replacement of 50% of the yolk of the embryonated hen's egg with large volumes of diverse but chemically defined solutions. By using an electrosurgical unit and a polyethylene tunnel, the procedure was performed on eggs from days 3 through 7 with greater than 90% surgical success and viability for the short term. More than 50% of the eggs replaced showed viability for 2 weeks, and a significant proportion went full term. (32)PO(4) and amino acids ((3)H and (14)C) added to the replaced eggs were incorporated into the macromolecules of the embryo and yolk sac as well as into parasitic rickettsiae cultivated in the replaced eggs. The incorporated (32)PO(4) was shown to be assimilated into a variety of biochemical species.

摘要

本文描述了一种技术，该技术可使用大量不同但化学成分明确的溶液替代孵化鸡胚50%的卵黄。通过使用电外科设备和聚乙烯隧道，在第3至7天的鸡蛋上进行该操作，短期手术成功率和存活率超过90%。超过50%被替换卵黄的鸡蛋存活了2周，并且有相当比例的鸡蛋发育至足月。添加到被替换鸡蛋中的(32)PO(4)和氨基酸（(3)H和(14)C）被整合到胚胎和卵黄囊的大分子中，以及在被替换鸡蛋中培养的寄生立克次氏体中。已证明掺入的(32)PO(4)被同化为多种生化物质。