Laitio M, Terho T
Lab Invest. 1975 Feb;32(2):183-9.
The polysaccharide composition of the human gallbladder well was studied in carcinomas and metaplastic changes of various degrees, and the results obtained were compared with those for the normal material previously presented (Terho, T., and Laitio, M. Biochim. Biophys. Acta 338: 135, 1974). Elevated amounts of acid connective tissue polysaccharides (heparitin and dermatan sulfates as well as chondroitin 4- or 6-sulfate, or both, could be observed in carinomas. In histochemical stainings it was found that in carcinomas and in the two specimens classified as group III (containing the most extensive metaplastic changes at disposal), the intracellular mucin was mainly neutral or nonsulfated acidic. The amounts of sulfated mucin were relatively insignificant. This mucin polysaccharide material was isolated and its composition was determined. It was observed to be large polysaccharide material was isolated and its composition was determined. It was observed to be large molecular (approximate molecular sizes 1 to 2 times 10-6), and to be composed of fucose, galactose, glucosamine, and galactosamine as well as small amounts of sialic acid. The basic structure of these polysaccharides is thus similar to that of normal sulfated mucin. The almost total absence of acid groups, however, causes the polysaccharide material in question to stain in a manner identical with neutral mucin when investigated with histochemical methods. The carcinomas also contained some sulfomucin; its proportion, however, was small as compared with the amounts of nonsulfated acid and neutral mucin in biochemical characterization. A small molecular polysaccharide fraction, assumed to originate in membrane-bound glycoproteins, was isolated from the insoluble gallbladder tissue residue. The proportion of this fraction was larger in carcinomas than in normal material. This rise as well as the rise in the quantity of acid connective tissue polysaccharides is presumably due to the large number of cells in the carcinoma tissue as well as to fibrosis.
对人类胆囊壁的多糖成分在各种程度的癌组织和化生改变中进行了研究，并将所得结果与之前所展示的正常材料的结果进行了比较（特尔霍，T.，和莱蒂奥，M.《生物化学与生物物理学报》338: 135，1974）。在癌组织中可观察到酸性结缔组织多糖（硫酸乙酰肝素、硫酸皮肤素以及硫酸软骨素4 - 或6 - 硫酸酯，或两者皆有）含量升高。在组织化学染色中发现，在癌组织以及被归类为III组的两个标本（包含可获得的最广泛化生改变）中，细胞内粘蛋白主要为中性或非硫酸化酸性。硫酸化粘蛋白的含量相对较少。对这种粘蛋白多糖物质进行了分离并确定了其组成。观察到它是大分子物质（近似分子大小为1至2×10⁻⁶），由岩藻糖、半乳糖、葡萄糖胺、半乳糖胺以及少量唾液酸组成。因此这些多糖的基本结构与正常硫酸化粘蛋白的结构相似。然而，几乎完全不存在酸性基团使得所讨论的多糖物质在用组织化学方法研究时以与中性粘蛋白相同的方式染色。癌组织中也含有一些硫粘蛋白；然而，与生化特征中未硫酸化酸性和中性粘蛋白的量相比，其比例较小。从不溶性胆囊组织残渣中分离出一个假定源自膜结合糖蛋白的小分子多糖部分。该部分在癌组织中的比例比正常材料中的大。这种增加以及酸性结缔组织多糖数量的增加可能是由于癌组织中的大量细胞以及纤维化所致。
