四膜虫中氯霉素抗性的细胞质遗传
Cytoplasmic inheritance of chloramphenicol resistance in tetrahymena.
作者信息
Roberts C T, Orias E
出版信息
Genetics. 1973 Feb;73(2):259-72. doi: 10.1093/genetics/73.2.259.
Abstract
Chloramphenicol-resistant mutants of Tetrahymena pyriformis were obtained after mutagenesis with nitrosoguanidine at an estimated frequency of 10(-3) mutants per mutagenized cell. The mutants are still partially sensitive to chloramphenicol and have a lowered growth rate, compared to the wild type strain, in rich medium without chloramphenicol. The genetic analysis described here indicates that chloramphenicol resistance is inherited as a cytoplasmic determinant that is not exchanged during conjugation. This represents the first simple cytoplasmic genetic determinant described in this species. A number of arguments favor a mitochondrial localization for this genetic determinant. In addition to the possible utility of such mutants for studies of mitochondrial structure and function in Tetrahymena, analogous mutations might serve as cytoplasmic "tags" in other ciliate species where the results of selfing need to be distinguished from those of outcrossing.
摘要
在用亚硝基胍诱变后，获得了梨形四膜虫氯霉素抗性突变体，估计诱变频率为每诱变细胞产生10⁻³个突变体。与野生型菌株相比，这些突变体对氯霉素仍有部分敏感性，且在不含氯霉素的丰富培养基中生长速率降低。此处描述的遗传分析表明，氯霉素抗性作为一种细胞质决定因素遗传，在接合过程中不发生交换。这是该物种中描述的第一个简单的细胞质遗传决定因素。许多论据支持该遗传决定因素定位于线粒体。除了这类突变体可能有助于研究梨形四膜虫的线粒体结构和功能外，类似的突变可能在其他纤毛虫物种中作为细胞质“标签”，在这些物种中，自体受精的结果需要与杂交的结果区分开来。
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