Abdul-Rahman A, Dahlgren N, Ingvar M, Rehncrona S, Siesjö B K
Acta Physiol Scand. 1979 May;106(1):53-60. doi: 10.1111/j.1748-1716.1979.tb06369.x.
Local cerebral blood flow (CBF) was measured in rats, using an autoradiographic technique with 14C-iodoantipyrine as diffusible tracer, in situations with low, normal and high flow rates (phenobarbital anesthesia, analgesia with 75% N2O, and hypoxia, respectively). A comparison of the results with previous data obtained in conscious rats (Sakurada et al. 1978) demonstrates that 75% N2O moderately reduces local CBF in some, but not all, cortical and subcortical areas, that phenobarbital anesthesia reduces local CBF to between 30 and 65% of (conscious) control, and that pronounced hypoxia (arterial P02 about 25 mmHg) increases local CBF 3- to 4-fold. A comparison of the values obtained for cortical structures with those previously measured with a technique based on the Fick principle shows that the autoradiographic technique gives similar values at low and normal flow rates but that it moderately underestimates CBF at high flow rates, probably due to diffusion limitation.
采用放射自显影技术，以¹⁴C-碘安替比林作为可扩散示踪剂，在低、正常和高血流速率情况下（分别为苯巴比妥麻醉、75%氧化亚氮镇痛和低氧）测量大鼠的局部脑血流量（CBF）。将这些结果与先前在清醒大鼠中获得的数据（Sakurada等人，1978年）进行比较表明，75%氧化亚氮会适度降低部分而非所有皮质和皮质下区域的局部CBF；苯巴比妥麻醉会将局部CBF降低至（清醒）对照的30%至65%；而明显的低氧（动脉血氧分压约为25 mmHg）会使局部CBF增加3至4倍。将通过放射自显影技术获得的皮质结构数值与先前基于菲克原理的技术所测量的数值进行比较表明，在低血流速率和正常血流速率下，放射自显影技术给出的数值相似，但在高血流速率下会适度低估CBF，这可能是由于扩散限制所致。
