卵泡发育对培养的猪颗粒细胞将雄激素转化为雌激素能力的影响。

Effects of follicular development on the ability of cultured porcine granulosa cells to convert androgens to estrogens.

作者信息

Anderson L D, Schaerf F W, Channing C P

出版信息

Adv Exp Med Biol. 1979;112:187-95. doi: 10.1007/978-1-4684-3474-3_22.

Abstract

Porcine granulosa cells from different stages of follicular development were examined for their ability to convert androgens to estrogens and their ability to secrete progesterone. Granulosa cells from all stages of follicular development can convert androgens to estrogens, and the addition of exogenous androgen is required for estrogen secretion. Granulosa cells obtained from medium and large follicles have a greater capacity to convert androgens to estrogens than do granulosa cells obtained from small follicles. The addition of FSH brought about an increase in estrogen secretion in the presence of androgen only in granulosa cells from large follicles. LH attenuated estrogen secretion in granulosa cells from medium follicles treated with testosterone. Granulosa cells from medium follicles were unable to secrete estrogen from days two to four irrespective of treatment. Androgens augment FSH stimulated progesterone secretion in granulosa cells from medium follicles from days two to four, and the addition of follicular fluid from small follicles stimulates progesterone secretion in the presence of FSH and androgens.

摘要

对来自卵泡发育不同阶段的猪颗粒细胞进行了检测，以研究它们将雄激素转化为雌激素的能力以及分泌孕酮的能力。卵泡发育各阶段的颗粒细胞均能将雄激素转化为雌激素，且雌激素分泌需要添加外源性雄激素。从中等大小和大卵泡获得的颗粒细胞比从小卵泡获得的颗粒细胞具有更强的将雄激素转化为雌激素的能力。仅在大卵泡的颗粒细胞中，添加促卵泡激素（FSH）会在有雄激素存在的情况下使雌激素分泌增加。促黄体生成素（LH）会减弱用睾酮处理的中等卵泡颗粒细胞中的雌激素分泌。在第2至4天，无论是否进行处理，中等卵泡的颗粒细胞都无法分泌雌激素。在第2至4天，雄激素会增强FSH刺激的中等卵泡颗粒细胞中的孕酮分泌，并且添加小卵泡的卵泡液会在有FSH和雄激素存在的情况下刺激孕酮分泌。