大鼠中神经垂体素的数量。用作示踪染料的溴酚蓝浓度对聚丙烯酰胺凝胶电泳分离蛋白质的影响。
The number of neurophysins in the rat. Influence of the concentration of Bromophenol Blue, used as a tracking dye, on the resolution of proteins by polyacrylamide-gel electrophoresis.
作者信息
Burford G D, Pickering B T
出版信息
Biochem J. 1972 Jul;128(4):941-4. doi: 10.1042/bj1280941.
Abstract
- The concentration of Bromophenol Blue used as tracking dye in polyacrylamide-gel electrophoresis affected the resolution of rat neurophysins. 2. A final dye concentration of 1mug/ml in the tris-glycine running buffer was found to give the best results. 3. The presence of two major and one minor neurophysin(s) in the rat was confirmed. 4. The two major proteins were found to re-run as single discrete bands, which had the same mobilities in the absence of dye and different mobilities in its presence.
摘要
- 用作聚丙烯酰胺凝胶电泳追踪染料的溴酚蓝浓度影响大鼠神经垂体激素运载蛋白的分离效果。2. 发现Tris-甘氨酸电泳缓冲液中染料终浓度为1μg/ml时效果最佳。3. 证实大鼠体内存在两种主要神经垂体激素运载蛋白和一种次要神经垂体激素运载蛋白。4. 发现这两种主要蛋白质重新电泳时呈现为单一离散条带，在无染料存在时具有相同迁移率，在有染料存在时具有不同迁移率。
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