用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
作者信息
Loening U E
出版信息
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
Abstract
- Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2. The mobility of RNA was inversely related to the sedimentation coefficient and varied with gel concentration. Electrophoresis in 2.2-2.6% gels gives a fractionation similar to density-gradient centrifugation. It shows the two ribosomal RNA components, the 45s precursor, transfer RNA and minor components. In 5% and 7.5% gels, 4s and 5s RNA separated and ribosomal RNA was excluded. 3. The resolution is greater and more detailed than by centrifugation, and many samples can be analysed simultaneously and rapidly.
摘要
- 凝胶由重结晶的丙烯酰胺和双丙烯酰胺制备而成。电泳在三羟甲基氨基甲烷 - 醋酸钠 - 乙二胺四乙酸缓冲液中进行0.5至3小时。凝胶在280或265毫微米处进行扫描。描述了切片和放射性计数的技术。2. RNA的迁移率与沉降系数成反比，并随凝胶浓度而变化。在2.2 - 2.6%的凝胶中进行电泳可得到类似于密度梯度离心的分级分离。它显示出两种核糖体RNA成分、45s前体、转移RNA和次要成分。在5%和7.5%的凝胶中，4s和5s RNA分离，核糖体RNA被排除在外。3. 其分辨率比离心法更高、更详细，并且可以同时快速分析许多样品。
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2
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