Plishker G A, Gitelman H J
Am J Physiol. 1979 Jul;237(1):C96-101. doi: 10.1152/ajpcell.1979.237.1.C96.
Calcium accumulation in intact human erythrocytes is enhanced by incubation in hypertonic solutions. Hypertonicity produces an increased permeability of the membrane to calcium that is time-dependent and occurs in the presence or absence of calcium. When hypertonically treated cells are incubated for more than 30 min in 300 mosmol/kg solutions the permeability of the membrane to calcium returns to basal values. Oligomycin inhibits the effect of hypertonicity on calcium uptake. The inhibitory action of oligomycin diminishes as the external sodium increases and can only be observed when the external concentration of potassium is at or below 3 mM. Low intracellular sodium and high intracellular potassium concentrations increase the uptake of calcium. It is concluded for human erythrocytes that 1) the increased permeability of the membrane to calcium produced by hypertonicity is a time-dependent, reversible phenomenon and is independent of calcium, 2) the increase in intracellular potassium concentration associated with hypertonic exposure is an important factor contributing to this response, and 3) interactions between calcium and components of the sodium-potassium transport system may account for the enhanced uptake of calcium produced by hypertonicity.
完整的人类红细胞在高渗溶液中孵育时，钙蓄积会增强。高渗状态会使细胞膜对钙的通透性增加，这种增加是时间依赖性的，且无论有无钙都可发生。当经高渗处理的细胞在300毫渗摩尔/千克的溶液中孵育超过30分钟时，细胞膜对钙的通透性会恢复到基础值。寡霉素会抑制高渗状态对钙摄取的影响。随着细胞外钠浓度增加，寡霉素的抑制作用会减弱，且只有当细胞外钾浓度等于或低于3毫摩尔时才能观察到这种抑制作用。低细胞内钠浓度和高细胞内钾浓度会增加钙摄取。对于人类红细胞可得出以下结论：1）高渗状态引起的细胞膜对钙通透性增加是一种时间依赖性的、可逆的现象，且与钙无关；2）与高渗暴露相关的细胞内钾浓度增加是导致这种反应的一个重要因素；3）钙与钠钾转运系统各组分之间的相互作用可能解释了高渗状态引起的钙摄取增加现象。
