Hsu I C, Smalley E B, Strong F M, Ribelin W E
Appl Microbiol. 1972 Nov;24(5):684-90. doi: 10.1128/am.24.5.684-690.1972.
Over a 5-month period during the winter of 1970-71, 20% of the lactating Holstein cows in a Wisconsin dairy herd died after prolonged ingestion of a diet containing 60% moldy corn infested with Fusarium tricinctum (2 x 10(5) propagules per g of moldy corn). Ethyl acetate extracts of the ground dried corn induced severe dermal reactions when applied to the skin of shaved 60-g albino rats and killed four of five 100-g rats that were force fed 1 ml in 2 ml of pure corn oil. T-2 toxin (3-hydroxy-4, 15-diacetoxy-8-[3-methylbutyryloxy]-12, 13-epoxy-Delta(9)-trichothecene) at concentrations of 2 mg per kg of dry corn was identified in purified extracts of the moldy corn by means of gas-liquid chromatography and mass spectrometry. This concentration of T-2 toxin in the moldy feed and the nature of the toxic effects observed strongly suggest a major causal relationship.
在1970 - 1971年冬季的5个月期间,威斯康星州一个奶牛场中20%的泌乳荷斯坦奶牛在长期摄入含有60%被三线镰刀菌侵染的发霉玉米(每克发霉玉米含2×10⁵个繁殖体)的日粮后死亡。将磨碎的干玉米的乙酸乙酯提取物涂抹在60克剃毛白化大鼠的皮肤上会引起严重的皮肤反应,并且在五只100克大鼠中,有四只被强迫灌喂2毫升纯玉米油中1毫升提取物后死亡。通过气液色谱法和质谱法在发霉玉米的纯化提取物中鉴定出浓度为每千克干玉米2毫克的T - 2毒素(3 - 羟基 - 4,15 - 二乙酰氧基 - 8 - [3 - 甲基丁酰氧基] - 12,13 - 环氧 - Δ⁹ - 单端孢霉烯)。发霉饲料中这种浓度的T - 2毒素以及所观察到的毒性作用性质强烈表明存在主要因果关系。