从荚膜组织胞浆菌酵母相分离一种新的可溶性抗原。
Isolation of a new soluble antigen from the yeast phase of Histoplasma capsulatum.
作者信息
Reeves M W, Pine L, Kaufman L, McLaughlin D
出版信息
Appl Microbiol. 1972 Nov;24(5):841-3. doi: 10.1128/am.24.5.841-843.1972.
Abstract
A method is described by which a soluble antigen was prepared from the yeast phase of Histoplasma capsulatum. This soluble preparation had a specificity greater than that of whole-cell yeast-phase antigens. In complement fixation tests with sera from human cases of histoplasmosis, blastomycosis, and coccidioidomycosis, the soluble antigen reacted in 12.1% of 141 tests with heterologous sera, whereas conventional whole-cell yeast antigens reacted in 47.3% of 91 tests with heterologous sera. The reactivities of the two types of antigens with homologous sera were essentially the same.
摘要
本文描述了一种从荚膜组织胞浆菌酵母相制备可溶性抗原的方法。这种可溶性制剂的特异性高于全细胞酵母相抗原。在用组织胞浆菌病、芽生菌病和球孢子菌病患者血清进行的补体结合试验中，可溶性抗原在141次异源血清试验中有12.1%呈反应，而传统的全细胞酵母抗原在91次异源血清试验中有47.3%呈反应。两种抗原与同源血清的反应性基本相同。
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