一种使用圆二色性测量的扁桃酸消旋酶直接动力学测定法。 - Suppr | 超能文献

A direct kinetic assay for mandelate racemase using circular dichroic measurements.

作者信息

Sharp T R, Hegeman G D, Kenyon G L

出版信息

Anal Biochem. 1979 Apr 15;94(2):329-34. doi: 10.1016/0003-2697(79)90368-3.

DOI:10.1016/0003-2697(79)90368-3

Abstract

摘要

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A direct kinetic assay for mandelate racemase using circular dichroic measurements.一种使用圆二色性测量的扁桃酸消旋酶直接动力学测定法。

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Selection and characterization of a mutant of the cloned gene for mandelate racemase that confers resistance to an affinity label by greatly enhanced production of enzyme.扁桃酸消旋酶克隆基因的一个突变体的筛选与鉴定，该突变体通过大量增加酶的产生而赋予对亲和标记物的抗性。

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