钙和锰离子对大鼠肝切片糖异生活性稳定性的研究。
A study of stabilization of gluconeogenic activity in rat liver slices by calcium and manganese ions.
作者信息
Roobol A, Alleyne G A
出版信息
Biochem J. 1972 Sep;129(2):231-9. doi: 10.1042/bj1290231.
Abstract
- The effect of some bivalent cations on gluconeogenesis by the rat liver-slice preparation has been investigated. 2. Ca(2+) and Mn(2+) stimulated glucose production from a range of substrates but not from glycerol. Mg(2+) had no effect on the rate of glucose production. 3. Ca(2+) were required to maintain phosphoenolpyruvate carboxylase activity in the slice preparation. 4. Ca(2+) and Mn(2+), but not Mg(2+), retarded the release of lysosomal enzymes from the slice into the incubation medium. 5. It is proposed that Ca(2+) and Mn(2+) stimulate glucose production by stabilizing the lysosome system in the liver-slice preparation. 6. The value of the liver-slice preparation as a means of measuring hepatic gluconeogenesis is discussed.
摘要
- 研究了某些二价阳离子对大鼠肝切片制备的糖异生作用。2. 钙离子（Ca(2+)）和锰离子（Mn(2+)）刺激了一系列底物生成葡萄糖，但对甘油生成葡萄糖无此作用。镁离子（Mg(2+)）对葡萄糖生成速率无影响。3. 需要钙离子（Ca(2+)）来维持切片制备中磷酸烯醇式丙酮酸羧化酶的活性。4. 钙离子（Ca(2+)）和锰离子（Mn(2+)），而非镁离子（Mg(2+)），抑制了溶酶体酶从切片释放到孵育培养基中。5. 有人提出，钙离子（Ca(2+)）和锰离子（Mn(2+)）通过稳定肝切片制备中的溶酶体系统来刺激葡萄糖生成。6. 讨论了肝切片制备作为测量肝糖异生手段的价值。
相似文献
1
A study of stabilization of gluconeogenic activity in rat liver slices by calcium and manganese ions.钙和锰离子对大鼠肝切片糖异生活性稳定性的研究。
Biochem J. 1972 Sep;129(2):231-9. doi: 10.1042/bj1290231.
2
Gluconeogenesis from D-glyceraldehyde and dihydroxyacetone in isolated rat liver. Stimulation by glucagon.离体大鼠肝脏中由D-甘油醛和二羟丙酮生成葡萄糖的过程。胰高血糖素的刺激作用。
Biochemistry. 1972 Aug 15;11(17):3286-9. doi: 10.1021/bi00767a025.
3
Inhibition of gluconeogenesis in isolated rat hepatocytes after chronic treatment with phenobarbital.苯巴比妥长期处理后对分离的大鼠肝细胞糖异生的抑制作用。
Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):663-9. doi: 10.1042/bj2800663.
4
Calcium, manganese and hepatic gluconeogenesis.钙、锰与肝脏糖异生作用
Biochim Biophys Acta. 1970 Oct 27;222(1):41-52. doi: 10.1016/0304-4165(70)90349-1.
5
Influence of L-tryptophan and its metabolites on gluconeogenesis in the isolated, perfused liver.L-色氨酸及其代谢产物对离体灌注肝脏糖异生的影响。
Biochemistry. 1967 Jul;6(7):2129-38. doi: 10.1021/bi00859a034.
6
The involvement of mitochondrial pyruvate transport in the pathways of gluconeogenesis from serine and alanine in isolated rat and mouse liver cells.线粒体丙酮酸转运在分离的大鼠和小鼠肝细胞中丝氨酸和丙氨酸糖异生途径中的作用。
FEBS Lett. 1975 Apr 15;53(1):29-32. doi: 10.1016/0014-5793(75)80674-0.
7
The interrelationship of the concentration of hydrogen ions, bicarbonate ions, carbon dioxide and calcium ions in the regulation of renal gluconeogenesis in the rat.大鼠肾糖异生调节中氢离子、碳酸氢根离子、二氧化碳和钙离子浓度的相互关系。
Biochem J. 1973 Nov;136(3):445-53. doi: 10.1042/bj1360445.
8
Stimulation by glucose of gluconeogenesis in hepatocytes isolated from starved rats.饥饿大鼠分离的肝细胞中葡萄糖对糖异生的刺激作用。
Biochem J. 1987 Aug 1;245(3):661-8. doi: 10.1042/bj2450661.
9
Carbohydrate formation from various precursors in noenatal rat liver.新生大鼠肝脏中由各种前体物质形成碳水化合物的过程。
Biochem J. 1968 Dec;110(4):725-31. doi: 10.1042/bj1100725.
10
Effect of bivalent cations on some enzymes of gluconeogenesis.二价阳离子对糖异生某些酶的影响。
Biochem J. 1970 Jun;118(2):19P. doi: 10.1042/bj1180019pa.
引用本文的文献
1
The interrelationship of the concentration of hydrogen ions, bicarbonate ions, carbon dioxide and calcium ions in the regulation of renal gluconeogenesis in the rat.大鼠肾糖异生调节中氢离子、碳酸氢根离子、二氧化碳和钙离子浓度的相互关系。
Biochem J. 1973 Nov;136(3):445-53. doi: 10.1042/bj1360445.
本文引用的文献
1
The rate of gluconeogenesis from various precursors in the perfused rat liver.在灌注的大鼠肝脏中，各种前体物质生成葡萄糖的速率。
Biochem J. 1967 Mar;102(3):942-51. doi: 10.1042/bj1020942.
2
Gluconeogenesis in mouse-liver slices.鼠肝切片中的糖异生作用。
Biochem J. 1966 Dec;101(3):607-17. doi: 10.1042/bj1010607.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.组织分级分离研究。4. 大鼠肝脏颗粒对酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶结合的比较研究。
Biochem J. 1955 Mar;59(3):433-8. doi: 10.1042/bj0590433.
5
PURIFICATION AND PROPERTIES OF HUMAN LIVER BETA-GLUCURONIDASE.人肝脏β-葡萄糖醛酸酶的纯化及性质
J Biol Chem. 1965 Jul;240:2811-6.
6
THE EFFECTS OF CORTICOSTEROIDS UPON CONNECTIVE TISSUE AND LYSOSOMES.皮质类固醇对结缔组织和溶酶体的作用。
Recent Prog Horm Res. 1964;20:215-45.
7
LABILIZATION AND STABILIZATION OF LYSOSOMES.溶酶体的易化与稳定
Fed Proc. 1964 Sep-Oct;23:1038-44.
8
Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices.肾糖异生。饮食对大鼠肾皮质切片糖异生能力的影响。
Biochem J. 1963 Jan;86(1):22-7. doi: 10.1042/bj0860022.
9
Studies of an vitro effect of glucocorticoids on gluconeogenesis.糖皮质激素对糖异生的体外作用研究。
Endocrinology. 1962 Sep;71:399-406. doi: 10.1210/endo-71-3-399.
10
Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose.葡萄糖氧化酶、过氧化物酶和邻联茴香胺在血液及尿液葡萄糖测定中的应用
Lancet. 1957 Aug 24;273(6991):368-70. doi: 10.1016/s0140-6736(57)92595-3.
Zotero 插件