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孕期尿液中孕二醇的快速分析。

Rapid analysis of pregnanediol in pregnancy urine.

作者信息

Cooper W, Coyle M G, Smith V K

出版信息

J Clin Pathol. 1972 Nov;25(11):933-9. doi: 10.1136/jcp.25.11.933.

DOI:10.1136/jcp.25.11.933
PMID:4648538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC477569/
Abstract

A simple, inexpensive, and rapid method for the determination of pregnanediol in pregnancy urine by gas liquid chromatography is described. Automatic injection of the samples into the gas chromatograph allows up to 36 samples to be run overnight thus saving valuable technical time.The method described can easily be adopted for use in a routine steroid laboratory. The results obtained by this method have been compared with values obtained by the method of Klopper, Michie, and Brown (1955).

摘要

本文描述了一种通过气液色谱法测定妊娠尿液中孕二醇的简单、廉价且快速的方法。将样品自动注入气相色谱仪，可在一夜之间分析多达36个样品，从而节省了宝贵的技术时间。所描述的方法可轻松应用于常规类固醇实验室。已将该方法获得的结果与Klopper、Michie和Brown（1955年）方法获得的值进行了比较。

相似文献

1
Rapid analysis of pregnanediol in pregnancy urine.孕期尿液中孕二醇的快速分析。
J Clin Pathol. 1972 Nov;25(11):933-9. doi: 10.1136/jcp.25.11.933.
2
Routine determination of urinary pregnanediol using a gas chromatograph with automatic sample application.使用带有自动进样装置的气相色谱仪对尿孕二醇进行常规测定。
J Clin Pathol. 1966 Nov;19(6):619-21. doi: 10.1136/jcp.19.6.619.
3
A semi-automated computerised gas-liquid chromatographic system for urinary steroid profile analysis.一种用于尿甾体谱分析的半自动计算机化气-液色谱系统。
J Clin Chem Clin Biochem. 1982 Dec;20(12):899-905. doi: 10.1515/cclm.1982.20.12.899.
4
The use of the steroid analyser in conjunction with a semi-automatic gas chromatograph in the routine analysis of urinary pregnanediol.在尿孕二醇的常规分析中，将类固醇分析仪与半自动气相色谱仪联合使用。
Clin Chim Acta. 1972 Jul;39(2):439-47. doi: 10.1016/0009-8981(72)90064-2.
5
A simple semi-automated gas-chromatographic method for determining pregnanediol and oestriol simultaneously in pregnancy urine.一种用于同时测定孕妇尿液中孕二醇和雌三醇的简单半自动气相色谱法。
Clin Chim Acta. 1972 Jul;39(2):449-54. doi: 10.1016/0009-8981(72)90065-4.
6
A simultaneous determination of pregnanetriol and pregnanediol by gas--liquid chromatography.气相色谱法同时测定孕三醇和孕二醇
Clin Chim Acta. 1974 Mar 26;51(3):233-40. doi: 10.1016/0009-8981(74)90308-8.
7
[Semi-automatic determination of urinary pregnandiol by gas-liquid chromatography].[气液色谱法半自动测定尿孕二醇]
Rev Clin Esp. 1974 Jul 15;134(1):17-22.
8
An improved method for the simultaneous determination of individual 17-oxosteroids and of pregnanediol in urine by gas-liquid chromatography.一种通过气液色谱法同时测定尿液中各17-氧类固醇和孕二醇的改进方法。
J Clin Chem Clin Biochem. 1977 Apr;15(4):225-11. doi: 10.1515/cclm.1977.15.1-12.225.
9
[Gaschromatographic method for routine determination of pregnanediol and pregnanetriol in urine (author's transl)].尿中孕二醇和孕三醇常规测定的气相色谱法（作者译）
J Clin Chem Clin Biochem. 1976 Jul;14(7):375-6.
10
[Simple routine determination of pregnanediol in urine during pregnancy by gas chromatography].[气相色谱法简单常规测定孕期尿中孕二醇]
Arch Gynakol. 1970 Apr 30;208(4):410-5. doi: 10.1007/BF00668255.

本文引用的文献

1
A method for the determination of urinary pregnanediol.一种测定尿孕二醇的方法。
J Endocrinol. 1955 May;12(3):209-19. doi: 10.1677/joe.0.0120209.
2
AN APPROACH TO THE SYSTEMATIC ANALYSIS OF URINARY STEROIDS.一种尿甾体系统分析方法
Biochem J. 1965 Apr;95(1):1-16. doi: 10.1042/bj0950001.
3
Gasphase chromatography in separation and identification of pregnanediol in urine and blood of pregnant women.气相色谱法用于孕妇尿液和血液中孕二醇的分离与鉴定。
Obstet Gynecol. 1963 Mar;21:302-7.
4
5beta-Pregnan-3alpha-ol-20-one in urine.尿液中的5β-孕烷-3α-醇-20-酮。
Acta Endocrinol (Copenh). 1962 Oct;41:247-58.
5
The value of the creatinine estimation as a gauge of the completeness of the 24-hour specimen.
J Obstet Gynaecol Br Emp. 1960 Aug;67:623-6. doi: 10.1111/j.1471-0528.1960.tb09224.x.
6
Some aspects of the urinary excretion of pregnanediol in pregnancy.孕期孕二醇的尿排泄的某些方面。
J Obstet Gynaecol Br Emp. 1959 Feb;66(1):1-11. doi: 10.1111/j.1471-0528.1959.tb01126.x.
7
Pregnanediol excretion in normal and abnormal pregnancy.正常与异常妊娠中孕二醇的排泄情况。
J Obstet Gynaecol Br Emp. 1957 Oct;64(5):649-67. doi: 10.1111/j.1471-0528.1957.tb08454.x.
8
A report on the chromatographic assay of urinary pregnanediol in pregnancy.孕期尿孕二醇色谱分析报告
J Obstet Gynaecol Br Emp. 1956 Aug;63(4):560-6. doi: 10.1111/j.1471-0528.1956.tb05532.x.
9
A simplified gas chromatographic method for large numbers of urinary pregnanediol determinations.一种用于大量尿孕二醇测定的简化气相色谱法。
Am J Obstet Gynecol. 1967 Jul 15;98(6):759-66. doi: 10.1016/0002-9378(67)90190-1.
10
Routine determination of urinary pregnanediol using a gas chromatograph with automatic sample application.使用带有自动进样装置的气相色谱仪对尿孕二醇进行常规测定。
J Clin Pathol. 1966 Nov;19(6):619-21. doi: 10.1136/jcp.19.6.619.