氧气和一氧化碳与马胃蝇血红蛋白结合的动力学
The kinetics of binding of oxygen and carbon monoxide to Gastrophilus haemoglobin.
作者信息
Phelps C F, Antonini E, Brunori M, Kellett G
出版信息
Biochem J. 1972 Oct;129(4):891-6. doi: 10.1042/bj1290891.
The dimeric haemoglobin in the tracheal cells of the Gastrophilus larva was extracted and purified, and the spectral properties of its oxy- and carbon monoxide adducts are recorded. In dilute solutions the kinetic parameters of binding of oxygen and carbon monoxide were determined. In solutions between 0.1 and 50mum for oxygen k(on) is 1x10(7)m(-1).s(-1) and k(off) is 1s(-1); for carbon monoxide l(on) is 6.5x10(5)m(-1).s(-1) and l(off) is 0.14s(-1). These values are in agreement with previous equilibrium results on oxygen binding and carbon monoxide/oxygen partition. These results are discussed and compared with the known values for other monomeric protohaem proteins.
提取并纯化了胃蝇幼虫气管细胞中的二聚体血红蛋白，并记录了其氧加合物和一氧化碳加合物的光谱特性。在稀溶液中测定了氧气和一氧化碳结合的动力学参数。在0.1至50μm的溶液中，氧气的k（on）为1×10⁷ m⁻¹·s⁻¹，k（off）为1 s⁻¹；一氧化碳的l（on）为6.5×10⁵ m⁻¹·s⁻¹，l（off）为0.14 s⁻¹。这些值与先前关于氧结合和一氧化碳/氧分配的平衡结果一致。对这些结果进行了讨论，并与其他单体原血红蛋白的已知值进行了比较。
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