用电子显微镜放射自显影术研究蜜蜂雄蜂视觉细胞中的蛋白质合成。
Protein synthesis in the visual cells of the honeybee drone as studied with electron microscope radioautography.
作者信息
Perrelet A
出版信息
J Cell Biol. 1972 Dec;55(3):595-605. doi: 10.1083/jcb.55.3.595.
Abstract
Protein synthesis was studied in the visual cells of an insect (honeybee drone, Apis mellifera) by electron microscope radioautography. After a single injection of tritiated leucine, the radioactivity first appears in the cytoplasm of the visual cell which contains ribosomes. Later, part of this radioactivity migrates to the rhabdome, the visual cell region which is specialized in light absorption. A maximal concentration of radioactivity is reached there 48 hr after the injection of leucine. This pattern of protein synthesis and transport resembles that described in vertebrate visual cells (rods and cones), where newly synthesized proteins have been shown to contribute to the renewal of the photoreceptor membrane.
摘要
通过电子显微镜放射自显影技术，对一种昆虫（蜜蜂雄蜂，Apis mellifera）的视觉细胞中的蛋白质合成进行了研究。单次注射氚标记的亮氨酸后，放射性首先出现在含有核糖体的视觉细胞的细胞质中。随后，部分这种放射性迁移到视杆，即视觉细胞中专门用于光吸收的区域。注射亮氨酸后48小时，视杆处达到放射性的最大浓度。这种蛋白质合成和运输模式类似于脊椎动物视觉细胞（视杆和视锥细胞）中所描述的模式，在脊椎动物视觉细胞中，新合成的蛋白质已被证明有助于光感受器膜的更新。
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