关于[甲硫氨酸5]-和[亮氨酸5]-脑啡肽在大鼠、豚鼠和小牛脑中的分布。
On the distribution of [met5]- and [leu5]-enkephalins in the brain of the rat, guinea-pig and calf.
作者信息
Di Giulio A M, Majane E M, Yang H Y
出版信息
Br J Pharmacol. 1979 Jun;66(2):297-301. doi: 10.1111/j.1476-5381.1979.tb13679.x.
Abstract
1 By means of a highly sensitive radioimmunoassay, the content of [met5]-enkephalin (ME) and [leu5]-enkephalin (LE) was measured in various regions of the rat, guinea-pig and calf brain. Provisions were made to differentiate ME from LE by the use of cyanogen bromide (CNBr) to destroy methionine, the carboxy terminal amino acid in the ME sequence. This allows for correction of possible errors due to the cross-reactivity of the ME to the LE antiserum. Evidence is presented to demonstrate that the specific radioimmunoassay combined with the CNBr technique is a valid method to measure LE and ME content in crude tissue extracts. 2 In all the species studied, enkephalins appeared to be highly concentrated in the striatum and hypothalamus while very low amounts were found in the cerebe-lum and hippocampus. 3 Although the ratio between ME and LE content varied from area to area, the ME content in every region of the rat, guinea-pig and calf brain was more than 4 fold greater than that of LE.
摘要
- 通过一种高度灵敏的放射免疫分析法，对大鼠、豚鼠和小牛大脑的各个区域中[甲硫氨酸5] - 脑啡肽（ME）和[亮氨酸5] - 脑啡肽（LE）的含量进行了测定。通过使用溴化氰（CNBr）破坏甲硫氨酸（ME序列中的羧基末端氨基酸）来区分ME和LE。这可以校正由于ME与LE抗血清的交叉反应可能导致的误差。有证据表明，结合CNBr技术的特异性放射免疫分析法是测量粗组织提取物中LE和ME含量的有效方法。2. 在所有研究的物种中，脑啡肽似乎高度集中在纹状体和下丘脑，而在小脑和海马体中含量极低。3. 虽然ME和LE含量的比例因区域而异，但大鼠、豚鼠和小牛大脑每个区域中的ME含量比LE含量高4倍以上。
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