一项通过凝胶电泳对毒蕈碱受体的研究。
A study of the muscarinic receptor by gel electrophoresis.
作者信息
Birdsall N J, Burgen A S, Hulme E C
出版信息
Br J Pharmacol. 1979 Jun;66(2):337-42. doi: 10.1111/j.1476-5381.1979.tb13685.x.
Abstract
1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.
摘要
- 来自大鼠、豚鼠和青蛙大脑的毒蕈碱受体已用氚标记的丙基苄基胆碱芥子碱（[³H]-PrBCM）进行标记。2. 溶解并经十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳后，可见一个单一的放射性标记峰，当在10⁻⁶ M阿托品存在下进行标记时，该峰被完全抑制。结果表明存在一条分子量类似于或约为80,000的单一多肽链。3. 在肠平滑肌的情况下，发现了多个标记峰。采取防止蛋白水解的措施后，鉴定出一个分子量在80,000左右的主要峰。
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本文引用的文献
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The binding of agonists to brain muscarinic receptors.激动剂与脑毒蕈碱受体的结合。
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