Shah P P, Tee A R, English P D, Redman B T, Bunyan J
Biochem J. 1972 Nov;130(2):467-73. doi: 10.1042/bj1300467.
The mass spectrum of a lipolytic substance from purified sheep midbrain extract indicated that it was isoprenaline, not N-n-propylnoradrenaline, noradrenaline or adrenaline. The identification of the lipid-mobilizing factor as isoprenaline was confirmed qualitatively by t.l.c. and g.l.c. and quantitatively by determination of its lipolytic activity and u.v. absorption and by fluorimetric determination of its catecholamine content.
纯化的绵羊中脑提取物中一种脂解物质的质谱表明,它是异丙肾上腺素,而非N-正丙基去甲肾上腺素、去甲肾上腺素或肾上腺素。通过薄层层析和气相色谱对作为异丙肾上腺素的脂质动员因子进行了定性确认,并通过测定其脂解活性、紫外吸收以及荧光法测定其儿茶酚胺含量进行了定量确认。