前列腺素与血浆蛋白的相互作用。前列腺素A2、F2和E2与人血浆蛋白的结合比较。
Interaction of prostaglandins with blood plasma proteins. Comparative binding of prostaglandins A 2 , F 2 and E 2 to human plasma proteins.
作者信息
Raz A
出版信息
Biochem J. 1972 Nov;130(2):631-6. doi: 10.1042/bj1300631.
Abstract
- The binding of prostaglandin A(2) and prostaglandin F(2alpha) to human plasma proteins was investigated by DEAE-Sephadex chromatography and polyacrylamide-gel electrophoresis. Both prostaglandins, when added to human plasma in vitro, were found to become bound mainly to plasma albumin. 2. The extent of binding of prostaglandins added to human plasma in low to moderate concentrations was found to be approx. 88, 73 and 58% for prostaglandins A(2), E(2) and F(2alpha) respectively. The order of affinities for the binding of the three prostaglandins to albumin appear to be A(2)>E(2)>F(2alpha). 3. The apparent association constants for the binding of these prostaglandins to human serum albumin were estimated to be approx. 4.8x10(4), 2.4x10(4) and 0.9x10(4) litre/mol for prostaglandins A(2), E(2) and F(2alpha) respectively. The results are compared with previously reported association constants for the binding of long-chain fatty acids to both human and bovine albumins.
摘要
- 通过二乙氨基乙基葡聚糖凝胶（DEAE - Sephadex）柱色谱法和聚丙烯酰胺凝胶电泳法研究了前列腺素A(2)和前列腺素F(2α)与人血浆蛋白的结合情况。当在体外将这两种前列腺素添加到人血浆中时，发现它们主要与血浆白蛋白结合。2. 发现以低至中等浓度添加到人血浆中的前列腺素的结合程度分别约为：前列腺素A(2)为88%，前列腺素E(2)为73%，前列腺素F(2α)为58%。这三种前列腺素与白蛋白结合的亲和力顺序似乎是A(2)>E(2)>F(2α)。3. 这些前列腺素与人血清白蛋白结合的表观缔合常数估计分别约为：前列腺素A(2)为4.8×10⁴升/摩尔，前列腺素E(2)为2.4×10⁴升/摩尔，前列腺素F(2α)为0.9×10⁴升/摩尔。将这些结果与先前报道的长链脂肪酸与人及牛白蛋白结合的缔合常数进行了比较。
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