Gamble C N
Am J Clin Pathol. 1975 Mar;63(3):310-7. doi: 10.1093/ajcp/63.3.310.
A simple, easily reproducible periodic acid-Schiff-light green stain (PAS-LG) for the detection of glomerular protein deposits by routine light microscopy is described. The deposits are selectively stained a deep blue and contrast sharply with the staining of adjacent glomerular structures. Correlation with immunofluorescent and electron microscopy has shown that it is possible with this stain to categorize accurately a large variety of glomerular lesions by light microscopy alone.
本文描述了一种简单、易于重现的过碘酸-希夫-淡绿染色法(PAS-LG),用于通过常规光学显微镜检测肾小球蛋白沉积物。这些沉积物被选择性地染成深蓝色,与相邻肾小球结构的染色形成鲜明对比。与免疫荧光和电子显微镜的相关性表明,仅通过这种染色法就有可能在光学显微镜下准确地对多种肾小球病变进行分类。