低试剂消耗的自动化补体结合试验。
Automated complement fixation with low reagent consumption.
作者信息
Olitzky I
出版信息
Appl Microbiol. 1972 Aug;24(2):208-12. doi: 10.1128/am.24.2.208-212.1972.
A microvolume automated (continuous-flow) system for the detection of complement-fixing antigen or antibody was evaluated. The model complement fixation test used was the detection of deoxyribonucleic acid antibody, and comparisons were made with a manual procedure. An "automatic two-dilution" feature of the apparatus was not used because of the clinical need for antibody titers. The system proved useful for the detection and estimation of anti-deoxyribonucleic acid antibody in patients with systemic lupus erythematosus and, indeed, accomplished this with low reagent consumption, a useful feature not available in "older" continuous-flow complement fixation systems.
对一种用于检测补体结合抗原或抗体的微量自动（连续流动）系统进行了评估。所使用的标准补体结合试验是检测脱氧核糖核酸抗体，并与手工操作进行了比较。由于临床需要检测抗体滴度，该仪器的“自动二次稀释”功能未被使用。该系统被证明可用于检测和评估系统性红斑狼疮患者的抗脱氧核糖核酸抗体，并且确实以低试剂消耗实现了这一点，这是“老式”连续流动补体结合系统所不具备的有用特性。
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