Tubular explant culture of fetal Syrian golden hamster tracheae.

Two types of organ culture technique (stationary and rocking) were compared for their ability to maintain the epithelial structures in the tubular explants of fetal Syrian golden hamster tracheae. A simple new technique has been devised for the organ culture of small tubular explants of fetal tracheae. A large pore-sized membrane filter is introduced into the rocking culture, which improves unfavourable in vitro effects of the stationary technique.