Siskind G W, Goidl E A
Fed Proc. 1975 Feb;34(2):151-2.
Neonatal liver or adult spleen was used as a source of B-lymphocytes in reconstituting lethally irradiated, syngeneic mice. Recipients were all given excess adult, syngeneic thymus cells and were immunized with dinitrophenylated bovine gamma globulin. The distribution of avidities of plaque-forming cells produced by immunized recipients of neonatal liver was highly restricted in comparison with animals reconstituted with adult spleen indicating a restriction of B-lymphocyte heterogeneity in the neonatal mouse.
在重建经致死剂量照射的同基因小鼠时，使用新生小鼠肝脏或成年小鼠脾脏作为B淋巴细胞的来源。所有受体均给予过量的成年同基因胸腺细胞，并用二硝基苯基化牛γ球蛋白进行免疫。与用成年脾脏重建的动物相比，接受新生小鼠肝脏免疫的受体产生的噬斑形成细胞亲和力分布受到高度限制，这表明新生小鼠的B淋巴细胞异质性受到限制。
