人血浆中前列腺素E、A和F的放射免疫分析测定
Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma.
作者信息
Jaffe B M, Behrman H R, Parker C W
出版信息
J Clin Invest. 1973 Feb;52(2):398-405. doi: 10.1172/JCI107196.
The details of a radioimmunoassay capable of measuring as 5 pg of prostaglandin A, E, and F (PGA, PGE, and PGF) in human and rat plasma are described. Plasma samples are extracted (with 4000 cpm [(3)H] PGE(1) added for calculation of recovery) with an organic solvent system at an apparent pH of 5.8 and then chromatographed on silicic acid columns with increasing concentrations of methanol to separate PGA, PGE, and PGF. Each chromatographed sample is measured by radioimmunoassay, using the homologous antibody and tritiated marker. 40 normal individuals had mean plasma concentrations of PGA, PGE, and PGF of 1062+/-107 pg/ml, 385+/-30 pg/ml, and 141+/-15 pg/ml, respectively. Elevated PGE levels were measured in the plasma of patients with medullary carcinoma of the thyroid, carcinoid, and neuroblastoma. Treatment of rats with indomethacin decreased serum PGE levels by 67%. The radioimmunoassay appears to be of considerable experimental as well as clinical interest.
本文描述了一种放射免疫分析法的详细情况，该方法能够测定人及大鼠血浆中低至5皮克的前列腺素A、E和F（PGA、PGE和PGF）。血浆样本用一种表观pH值为5.8的有机溶剂系统进行提取（加入4000计数每分钟的[(3)H]PGE(1)用于回收率计算），然后在硅酸柱上用浓度递增的甲醇进行色谱分离，以分离PGA、PGE和PGF。每个经色谱分离的样本都采用放射免疫分析法进行测定，使用同源抗体和氚标记物。40名正常个体的血浆中PGA、PGE和PGF的平均浓度分别为1062±107皮克/毫升、385±30皮克/毫升和141±15皮克/毫升。在甲状腺髓样癌、类癌和神经母细胞瘤患者的血浆中检测到PGE水平升高。用消炎痛治疗大鼠可使血清PGE水平降低67%。该放射免疫分析法似乎具有相当大的实验及临床意义。
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