Yu S C, Chang L, Burke G
J Clin Invest. 1972 Apr;51(4):1038-42. doi: 10.1172/JCI106864.
We have shown that two unrelated prostaglandin antagonists block both thyrotropin (TSH) and prostaglandins E (PGE(1), PGE(2)) stimulation of thyroidal adenyl cyclase activation and cyclic 3',5'-adenosine monophosphate (cAMP) formation, suggesting that prostaglandins play an important role in regulating thyroid function. To further explore this postulate, we measured prostaglandin content by radioimmunoassay in homogeneous bovine thyroid cell preparations in the presence and absence of TSH. Antibodies to albumin-conjugated PGE(1) and PGF(2alpha) showed specificity for prostaglandins E and F, respectively, but reacted, albeit far less effectively, with heterologous prostaglandins. A double antibody system was used to separate free from antibody-bound PGE(1)-(3)H and PGF(2alpha)-(3)H. Thyroid cells were extracted with ethanol/ethyl acetate and the various prostaglandins separated on silicic acid columns. Recoveries of added PGE(1)-(3)H and PGF(2alpha)-(3)H through the extraction and separation procedures ranged from 50-80%. The sensitivity of the method was 10-50 pg. Basal thyroid cell content of PGE(1) and PGF(2alpha) "equivalents" varied between cell preparations (range = 2-6 ng/0.2 ml cell suspension) but, in each instance, remained constant during 5-30-min incubations at 37 degrees C. TSH, 10-100 mU/ml, increased the levels of cell PGE(1) and PGF(2alpha) "equivalents" 30-80% above basal during 5-15-min incubations. The stimulatory effect was specific for TSH, no increase in PGE(1) or PGF(2alpha) "equivalent" levels being seen with luteinizing hormone (LH), human growth hormone (HGH), adrenocorticotropic hormone (ACTH), or glucagon. These data support the thesis that prostaglandins may mediate TSH effects on thyroid.
我们已经表明，两种不相关的前列腺素拮抗剂可阻断促甲状腺素（TSH）和前列腺素E（PGE₁、PGE₂）对甲状腺腺苷酸环化酶激活和环磷酸腺苷（cAMP）形成的刺激作用，这表明前列腺素在调节甲状腺功能中起重要作用。为了进一步探讨这一假设，我们在有和没有TSH的情况下，通过放射免疫测定法在均匀的牛甲状腺细胞制剂中测量了前列腺素含量。针对白蛋白偶联的PGE₁和PGF₂α的抗体分别对前列腺素E和F具有特异性，但与异源前列腺素的反应较弱。使用双抗体系统分离游离的和与抗体结合的PGE₁-³H和PGF₂α-³H。用乙醇/乙酸乙酯提取甲状腺细胞，并在硅酸柱上分离各种前列腺素。通过提取和分离程序添加的PGE₁-³H和PGF₂α-³H的回收率在50%-80%之间。该方法的灵敏度为10-50 pg。PGE₁和PGF₂α“等效物”的基础甲状腺细胞含量在不同细胞制剂之间有所不同（范围为2-6 ng/0.2 ml细胞悬液），但在每种情况下，在37℃孵育5-30分钟期间保持恒定。10-100 mU/ml的TSH在5-15分钟孵育期间使细胞PGE₁和PGF₂α“等效物”水平比基础水平增加30%-80%。这种刺激作用对TSH具有特异性，促黄体生成素（LH）、人生长激素（HGH）、促肾上腺皮质激素（ACTH）或胰高血糖素不会使PGE₁或PGF₂α“等效物”水平增加。这些数据支持前列腺素可能介导TSH对甲状腺的作用这一论点。
