豚鼠对青霉素-自体载体蛋白的免疫反应。
Immune response in the guinea pig to penicillin-autologous carrier proteins.
作者信息
Girard J P, Cuevas M
出版信息
Int Arch Allergy Appl Immunol. 1975;48(3):422-8. doi: 10.1159/000231327.
The spontaneous fixation of penicillin G on guinea pig serum protein was studied. Serum albumin and Ig were shown to fix firmly over 95% of penicillin. Therefore, guinea pigs were immunized with penicillin alone or penicillin-autologous carrier protein conjugates. The production of hemagglutinating antibody, of MIF and the in vitro stimulation of lymphocytes were analyzed as immunological parameters. The results showed that in all cases the strongest immunological response was obtained with animals immunized with the protein-hapten conjugates, whereas no response could be obtained with the carrier protein alone. Finally it was shown that the conjugates significantly stimulated purified T cell population. These results suggest that the antigenic recognition of penicillin involves the participation of lymphocytes.
研究了青霉素G在豚鼠血清蛋白上的自发结合。结果表明，血清白蛋白和免疫球蛋白能牢固结合超过95%的青霉素。因此，用青霉素单独或青霉素-自体载体蛋白偶联物对豚鼠进行免疫。分析了血凝抗体、巨噬细胞移动抑制因子（MIF）的产生以及淋巴细胞的体外刺激情况作为免疫参数。结果显示，在所有情况下，用蛋白-半抗原偶联物免疫的动物获得了最强的免疫反应，而单独使用载体蛋白则未获得反应。最后表明，这些偶联物能显著刺激纯化的T细胞群体。这些结果提示，青霉素的抗原识别涉及淋巴细胞的参与。
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