Lai M, Lampert I A, Lewis P D
Histochemistry. 1975;41(3):275-9. doi: 10.1007/BF00497691.
Cytoplasmic staining of glial cells by Alcian blue in 0.40 M magnesium chloride can be demonstrated in sections of brain tissue fixed in formol-acetic acid and in Bouin's solution. Phosphate-buffered aldehyde fixatives, whether at neutral or low pH, fail to preserve stainable material.