Turley S D, West C E, Horton B J
Lipids. 1976 Apr;11(4):281-6. doi: 10.1007/BF02544054.
The relative rates of sterol synthesis in the liver, ileum, and lung of the guinea pig have been studied by measuring the incorporation by tissue slices of 14C-labeled acetate into digitonin-precipitable sterols. The liver showed maximum incorporation of acetate at pH 6.5, the ileum at pH 7.5, and the lung at pH 6.0. The incorporation of acetate approached the maximum rate at a concentration of 10 mM with the liver and lung and 5 mM with the ileum. Using these conditions of assay, sterol synthesis was measured in the liver, ileum, and lung of four groups of guinea pigs killed at 6-hourly intervals. Depending on the time of day, the rate of sterol synthesis in the ileum was from 6 to 14 times that in the liver, while in the lung the rate was up to 3 times that shown by the liver, Additional studies showed that all regions of the small intestine synthesized sterol at a higher rate than the liver, with the highest rate of synthesis occurring in the ileum. The rates observed in the adrenal, testis, muscle, adipose tissue, and skin indicated that these tissues are not quantitatively important sites of sterol synthesis in the guinea pig.
通过测量豚鼠肝脏、回肠和肺组织切片将14C标记的乙酸盐掺入可被洋地黄皂苷沉淀的甾醇中的情况，研究了这些组织中甾醇合成的相对速率。肝脏在pH 6.5时乙酸盐掺入量最高，回肠在pH 7.5时最高，肺在pH 6.0时最高。肝脏和肺在乙酸盐浓度为10 mM时、回肠在乙酸盐浓度为5 mM时，乙酸盐掺入接近最大速率。在这些测定条件下，对四组每隔6小时处死的豚鼠的肝脏、回肠和肺中的甾醇合成进行了测量。根据一天中的时间不同，回肠中甾醇合成速率是肝脏中的6至14倍，而肺中的速率高达肝脏的3倍。进一步研究表明，小肠所有区域的甾醇合成速率均高于肝脏，其中回肠的合成速率最高。在肾上腺、睾丸、肌肉、脂肪组织和皮肤中观察到的速率表明，这些组织在豚鼠体内并非甾醇合成的重要定量部位。
