Shin M L, Firminger H I
Am J Pathol. 1973 Mar;70(3):291-313.
Malignant mesotheliomas were induced in the rat peritoneum by a single injection of chrysotile or crocidolite asbestos fibers. The immediate toxicity of the fibers was noted in both groups of animals, producing approximately 40% mortality, within 8 days after the injection associated with acute peritonities. Tissue reactions to these two types of asbestos were significantly different. Crocidolite fibers were easily seen by light microscopy, in the tissue sections throughout the period of study, and they produced foreign-body giant cell granulomas. However, giant cells were not seen in chrysotile granulomas, and the asbestos fibers were only seen by electron microscopic study. They appeared to be coated by a protein-like substance. During earlier stages of tumorigenesis, the epithelioid and/or mixed cell type mesotheliomas seemed to have no specific relationship to granulomas, but pure spindle cell tumors were seen to develop in close relationship to granulomas, and they appear to be fibrosarcomas. Electron microscopic and histochemical methods were used to define the morphologic characteristics of the tumor cells. The formation of hyaluronic acid was found in cells of the epithelioid type, contrasted with extracellular accumulation in the spindle cell tumors.
通过单次注射温石棉或青石棉纤维在大鼠腹膜中诱发恶性间皮瘤。两组动物均出现纤维的即时毒性，注射后8天内产生约40%的死亡率，伴有急性腹膜炎。对这两种类型石棉的组织反应显著不同。在整个研究期间，在组织切片中通过光学显微镜很容易看到青石棉纤维，它们产生异物巨细胞肉芽肿。然而，在温石棉肉芽肿中未见巨细胞，石棉纤维仅通过电子显微镜研究才能看到。它们似乎被一种蛋白质样物质包裹。在肿瘤发生的早期阶段，上皮样和/或混合细胞型间皮瘤似乎与肉芽肿没有特定关系，但纯梭形细胞瘤与肉芽肿密切相关，且它们似乎是纤维肉瘤。使用电子显微镜和组织化学方法来确定肿瘤细胞的形态特征。发现上皮样细胞类型的细胞中形成透明质酸，这与梭形细胞瘤中的细胞外积累形成对比。
