Institute of Occupational Medicine, Roxburgh Place, Edinburgh, UK.
Carcinogenesis. 1980 Mar;1(3):219-31. doi: 10.1093/carcin/1.3.219.
Cell lines were established in vitro from five peritoneal mesotheliomas produced in rats by the injection of crocidolite asbestos. These tumours exhibited the previously described diverse range of histological patterns normally associated with mesotheliomas. This diversity of appearance was also evident in culture but cell patterns in vitro did not necessarily correspond to the histology of the original tumour. With increased time in culture most cell lines tended towards a pattern of random orientation associated with the piling up of cells to form discrete masses. Despite this variation in cell pattern in culture, few ultrastructural differences were seen during electron microscope examination. Following varying periods in culture, some cell lines from these tumours were injected into either rats or athymic mice. Only one typical rat mesothelioma was produced but all cell lines produced tumours in athymic mice. The implications of these findings are discussed in relation to the aetiology of asbestos induced mesotheliomas.
从用青石棉注射诱发的 5 例大鼠腹膜间皮瘤中建立了体外细胞系。这些肿瘤表现出先前描述过的通常与间皮瘤有关的多种多样的组织学类型。这种外观的多样性在培养中也很明显，但体外细胞类型并不一定与原始肿瘤的组织学相对应。随着培养时间的延长，大多数细胞系趋向于一种与细胞堆积形成离散肿块有关的随机定向模式。尽管在培养过程中细胞形态存在差异，但在电子显微镜检查中很少观察到超微结构的差异。在培养的不同时期后，将这些肿瘤的一些细胞系注射到大鼠或无胸腺小鼠中。仅产生了 1 种典型的大鼠间皮瘤，但所有细胞系均在无胸腺小鼠中产生了肿瘤。这些发现的意义与石棉诱发间皮瘤的病因学有关。
